-
Product Name
TIPE2 antibody
- Documents
-
Description
TIPE2 Rabbit Polyclonal antibody. Positive IP detected in mouse cerebellum tissue. Positive WB detected in mouse cerebellum tissue, mouse small intestine tissue, mouse spleen tissue, rat lymph tissue, rat spleen tissue. Positive IHC detected in human lymphoma tissue. Positive IF detected in HepG2 cells. Observed molecular weight by Western-blot: 18 kDa
-
Tested applications
ELISA, WB, IHC, IP, IF
-
Species reactivity
Human,Mouse,Rat; other species not tested.
-
Alternative names
FLJ23467 antibody; Inflammation factor protein 20 antibody; TIPE2 antibody; TNFAIP8 like protein 2 antibody; TNFAIP8L2 antibody
- Immunogen
-
Isotype
Rabbit IgG
-
Preparation
This antibody was obtained by immunization of TIPE2 recombinant protein (Accession Number: NM_024575). Purification method: Antigen affinity purified.
-
Clonality
Polyclonal
-
Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
-
Storage instructions
Store at -20℃. DO NOT ALIQUOT
-
Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:10-1:100
-
Validations
mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with Catalog No:116064(TNFAIP8L2 antibody) at dilution of 1:500
Immunohistochemical of paraffin-embedded human lymphoma using Catalog No:116064(TNFAIP8L2 antibody) at dilution of 1:50 (under 40x lens)
IP Result of anti-TNFAIP8L2 (IP:Catalog No:116064, 3ug; Detection:Catalog No:116064 1:500) with mouse cerebellum tissue lysate 4000ug.
Immunofluorescent analysis of HepG2 cells using Catalog No:116064(TNFAIP8L2 Antibody) at dilution of 1:25 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
-
Background
TIPE2 (TNF-α-inducible protein 8-like 2, or TNFAIP8L2), a negative immune regulator, is essential for maintaining immune homeostasis. TIPE2 shares considerable sequence homology with members of the tumor necrosis factor-α-inducible protein 8 (TNFAIP8) family, which are thought to regulate cellular and immune homeostasis. TIPE2 acts as a negative regulator of Toll-like receptor and T-cell receptor function and is preferentially expressed in lymphoid tissues. TIPE2 plays an essential role for a signal transduction pathway that links inflammatory immune response to specific conditions after cerebral ischemia
-
References
- Zhang S, Zhang Y, Wei X. Expression and regulation of a novel identified TNFAIP8 family is associated with diabetic nephropathy. Biochimica et biophysica acta. 1802(11):1078-86. 2010.
- Zhang Y, Wei X, Liu L. TIPE2, a novel regulator of immunity, protects against experimental stroke. The Journal of biological chemistry. 287(39):32546-55. 2012.
- Li J, Liu K, Liu Y. Exosomes mediate the cell-to-cell transmission of IFN-α-induced antiviral activity. Nature immunology. 14(8):793-803. 2013.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"