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Product Name
Thymidylate synthase antibody
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Description
Thymidylate synthase Rabbit Polyclonal antibody. Positive IF detected in HepG2 cells. Positive IHC detected in human endometrial cancer tissue, human colon cancer tissue. Positive WB detected in HeLa cells, C6 cells, NIH/3T3 cells. Positive IP detected in HeLa cells. Observed molecular weight by Western-blot: 32-36kd
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Tested applications
ELISA, WB, IHC, IF, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
HsT422 antibody; Thymidylate synthase antibody; thymidylate synthetase antibody; TMS antibody; TSase antibody; TYMS antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of Thymidylate synthase recombinant protein (Accession Number: NM_001071). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:116115(TYMS antibody) at dilution of 1:1500
Immunohistochemical of paraffin-embedded human endometrial cancer using Catalog No:116115(TYMS antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human endometrial cancer using Catalog No:116115(TYMS antibody) at dilution of 1:100 (under 40x lens)
Immunofluorescent analysis of HepG2 cells, using TYMS antibody Catalog No:116115 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
IP Result of anti-TYMS (IP:Catalog No:116115, 3ug; Detection:Catalog No:116115 1:600) with HeLa cells lysate 3200ug.
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Background
TYMS represents thymidylate synthetase
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References
- Liao B, Liang H, Chen J, Liu Q, Zhang B, Chen X. Suberoylanilide hydroxamic acid enhances chemosensitivity to 5-fluorouracil in hepatocellular carcinoma via inhibition of thymidylate synthase. Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine. 36(12):9347-56. 2015.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"