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Product Name
Thrombospondin-1 antibody
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Description
Thrombospondin-1 Rabbit Polyclonal antibody. Positive WB detected in HeLa cells, A431 cells, A549 cells, human heart tissue. Positive IP detected in HeLa cells. Positive IF detected in MCF-7 cells. Observed molecular weight by Western-blot: 165-198 kDa
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Tested applications
ELISA, WB, IF, IP
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Species reactivity
Human; other species not tested.
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Alternative names
THBS antibody; THBS 1 antibody; THBS1 antibody; thrombospondin 1 antibody; TSP antibody; TSP 1 antibody; TSP1 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of Peptide (Accession Number: BC028145). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:2000
IF: 1:20-1:200
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:116056(TSP1 antibody) at dilution of 1:300
IP Result of anti-TSP1 (IP:Catalog No:116056, 4ug; Detection:Catalog No:116056 1:500) with HeLa cells lysate 2000ug.
Immunofluorescent analysis of MCF-7 cells using Catalog No:116056(TSP1 Antibody) at dilution of 1:50 and Rhodamine-Goat anti-Rabbit IgG
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Background
TSP1, also named as THBS1, THBS and TSP, is an adhesive glycoprotein that mediates cell-to-cell and cell-to-matrix interactions, and which can bind to fibrinogen, fibronectin, laminin, type V collagen and integrins alpha-V/beta-1, alpha-V/beta-3 and alpha-IIb/beta-3. TSP1 is a regulator of many biological processes including cell growth, adhesion, migration, platelet aggregation, and fibrin deposition and lysis. It interacts with a number of plasma proteins such as fibrinogen and plasminogen and co-polymerizes with fibrin in clot formation.
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References
- Xu J, Cai H, Meng Q. IL-1β-regulating angiogenic factors expression in perforated temporomandibular disk cells via NF-κB pathway. Journal of oral pathology & medicine : official publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology. 2016.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"