Thrombospondin-1 (1016-1023) (human, bovine, mouse) peptide
Not For Human Use, Lab Use Only.
Cat.#: 302561
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TFA RemovalWhat's this?
Special Price 101.2 USD
Availability:
1-2 weeks
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Product Name
Thrombospondin-1 (1016-1023) (human, bovine, mouse) peptide
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Documents
Batch to batch variation of the purity
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Sequence Shortening
RFYVVMWK
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Sequence
H-Arg-Phe-Tyr-Val-Val-Met-Trp-Lys-OH
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Length (aa)
8
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Peptide Purity (HPLC)
97.7%
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Molecular Formula
C56H81N13O10S
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Molecular Weight
1128.41
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CAS No.
149234-04-8
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Source
Synthetic
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Form
Powder
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Description
The octapeptide RFYVVMWK constitutes the active sequence within the 30-mer peptide named C4 as it is essential for the cell attachment activity of the TS1 CBD. It supports the attachment of G361 melanoma cells and inhibits their adhesion to the rec CBD of TS1. Moreover, this peptide appears to be highly conserved in all TS1 isoforms, and a related sequence is also present in the fragment F9 of laminin. H-1414 (4N1) has been used as CD47 agonist (see also H-6414).
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Storage Guidelines
Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual: Handling and Storage of Synthetic Peptides
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References
- M.D.Kosfeld et al., J. Biol. Chem., 266, 24257 (1991)
- M.D.Kosfeld and W.A.Frazier, J. Biol. Chem., 267, 16230 (1992)
- M.D.Kosfeld and W.A.Frazier, J. Biol. Chem., 268, 8808 (1993)
- A.Saumet et al., Blood, 106, 658 (2005)
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About TFA salt
Trifluoroacetic acid (TFA) has a significant impact on peptides due to its role in the peptide synthesis process.
TFA is essential for the protonation of peptides that lack basic amino acids such as Arginine (Arg), Histidine (His), and Lysine (Lys), or ones that have blocked N-termini. As a result, peptides often contain TFA salts in the final product.
TFA residues, when present in custom peptides, can cause unpredictable fluctuations in experimental data. At a nanomolar (nM) level, TFA can influence cell experiments, hindering cell growth at low concentrations (as low as 10 nM) and promoting it at higher doses (0.5–7.0 mM). It can also serve as an allosteric regulator on the GlyR of glycine receptors, thereby increasing receptor activity at lower glycine concentrations.
In an in vivo setting, TFA can trifluoroacetylate amino groups in proteins and phospholipids, inducing potentially unwanted antibody responses. Moreover, TFA can impact structure studies as it affects spectrum absorption.
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Molar Concentration Calculator
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Dilution Calculator
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Percent Concentration Calculator
Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)
Peptide Property
- Analysed Sequence:H-RFYVVMWK-OH
- Chemical Formula:C56H81N13O10S
- Sequence length:8
- Extinction coefficient:6970 M-1cm-1
- GRAVY:0.31
- Mw average:1128.38
- Theoretical pI:10.39
- Data Source:Peptide Property Calculator
GRAVY = grand average of hydropathy
X: Hydrophobic uncharged residues, like F I L M V W A and P
X: Basic residues, like R K H
X: Acidic residues, like D E
X: Polar uncharged residues, like G S T C N Q and Y
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"