Human Thioredoxin/TXN/SASP recombinant protein

Human Thioredoxin/TXN/SASP recombinant protein

• DataSheet
• Material Safety Data Sheets (MSDS)

Participates in various redox reactions through the reversible oxidation of its active center dithiol to a disulfide and catalyzes dithiol-disulfide exchange reactions. Plays a role in the reversible S-nitrosylation of cysteine residues in target proteins, and thereby contributes to the response to intracellular nitric oxide. Nitrosylates the active site Cys of CASP3 in response to nitric oxide (NO), and thereby inhibits caspase-3 activity. Induces the FOS/JUN AP-1 DNA-binding activity in ionizing radiation (IR) cells through its oxidation/reduction status and stimulates AP-1 transcriptional activity.

Thioredoxin

ADF; TRX; THIOREDOXIN; TRX1; MGC61975; DKFZP686B1993; RP23-332H16.1; TXN1; RP11-427L11.1; TXN; AW550880

P10599

TXN; TRDX; TRX; TRX1

E. coli

A DNA sequence encoding the C-terminal segment of human TXN (P10599) (Met 1-Val 105) was expressed and purified.

Human

The recombinant human TXN comprises 105 amino acids and predicts a molecular mass of 11.7 kDa. It migrates ass an approximately 14 kDa band in SDS-PAGE under reducing conditions.

> 97 % as determined by SDS-PAGE

1. Measured by its ability to catalyze the reduction of insulin. The specific activity is 5-9 pmoles/min/μg.
2. Measured by its ability to catalyze the reduction of insulin. The reaction leads toprecipitation, which can be measured by absorbance at 650 nm. The specific activity is 5-10 A650/min/mg.