Product Name

Thioredoxin antibody

Documents

Description

Thioredoxin Rabbit Polyclonal antibody. Positive WB detected in Jurkat cells, A2780 cells, HeLa cells, human brain tissue, human liver tissue, mouse liver tissue, rat brain tissue. Positive IP detected in Jurkat cells. Positive FC detected in HeLa cells. Positive IHC detected in human liver tissue, human cervical cancer tissue, human skeletal muscle tissue. Positive IF detected in Hela cells. Observed molecular weight by Western-blot: 12 kDa

Tested applications

ELISA, IHC, IF, FC, IP, WB

Species reactivity

Human,Mouse,Rat; other species not tested.

Alternative names

ADF antibody; ATL derived factor antibody; DKFZp686B1993 antibody; SASP antibody; Thioredoxin antibody; TRDX antibody; TRX antibody; TRX1 antibody; TXN antibody

Isotype

Rabbit IgG

Preparation

This antibody was obtained by immunization of Thioredoxin recombinant protein (Accession Number: NM_003329). Purification method: Antigen affinity purified.

Clonality

Polyclonal

Formulation

PBS with 0.02% sodium azide and 50% glycerol pH 7.3.

Storage instructions

Store at -20℃. DO NOT ALIQUOT

Applications

Recommended Dilution:

WB: 1:1000-1:10000

IP: 1:200-1:1000

IHC: 1:20-1:200

IF: 1:20-1:200

Validations

Immunohistochemical of paraffin-embedded human liver using Catalog No:116044(TXN antibody) at dilution of 1:100 (under 10x lens)

Immunohistochemical of paraffin-embedded human liver using Catalog No:116044(TXN antibody) at dilution of 1:100 (under 40x lens)

Immunofluorescent analysis of Hela cells, using TXN antibody Catalog No:116044 at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

Jurkat cells were subjected to SDS PAGE followed by western blot with Catalog No:116044(TXN Antibody) at dilution of 1:2000

IP Result of anti-TXN (IP:Catalog No:116044, 4ug; Detection:Catalog No:116044 1:300) with Jurkat cells lysate 2000ug.

1X10^6 HeLa cells were stained with 0.2ug TXN antibody (Catalog No:116044, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

Background

TXN, TRDX, TRX, TRX1, ADF and SASP, belongs to the thioredoxin family. It participates in various redox reactions through the reversible oxidation of its active center dithiol to a disulfide and catalyzes dithiol-disulfide exchange reactions. TXN plays a role in the reversible S-nitrosylation of cysteine residues in target proteins, and thereby contributes to the response to intracellular nitric oxide. Nitrosylates the active site Cys of CASP3 in response to nitric oxide (NO), and thereby inhibits caspase-3 activity. TXN induces the FOS/JUN AP-1 DNA-binding activity in ionizing radiation (IR) cells through its oxidation/reduction status and stimulates AP-1 transcriptional activity.

References

Ma Z, Zhang H, Zheng J. Interaction between M-like protein and macrophage thioredoxin facilitates antiphagocytosis for Streptococcus equi ssp. zooepidemicus. PloS one. 7(2):e32099. 2012.
Wu H, Xiao Y, Zhang S. The Ets transcription factor GABP is a component of the hippo pathway essential for growth and antioxidant defense. Cell reports. 3(5):1663-77. 2013.
Zhao H, Wang R, Tao Z. Ischemic postconditioning relieves cerebral ischemia and reperfusion injury through activating T-LAK cell-originated protein kinase/protein kinase B pathway in rats. Stroke; a journal of cerebral circulation. 45(8):2417-24. 2014.
Brown-Borg HM, Rakoczy S, Wonderlich JA, Armstrong V, Rojanathammanee L. Altered dietary methionine differentially impacts glutathione and methionine metabolism in long-living growth hormone-deficient Ames dwarf and wild-type mice. Longevity & healthspan. 3(1):10. 2014.
Zhu X, Huang C, Peng B. Overexpression of thioredoxin system proteins predicts poor prognosis in patients with squamous cell carcinoma of the tongue. Oral oncology. 47(7):609-14. 2011.

Thioredoxin antibody

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