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Product Name
Human TGFBR2 (His & Fc Tag) recombinant protein
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Description
Transmembrane serine/threonine kinase forming with the TGF-beta type I serine/threonine kinase receptor, TGFBR1, the non-promiscuous receptor for the TGF-beta cytokines TGFB1, TGFB2 and TGFB3. Transduces the TGFB1, TGFB2 and TGFB3 signal from the cell surface to the cytoplasm and is thus regulating a plethora of physiological and pathological processes including cell cycle arrest in epithelial and hematopoietic cells, control of mesenchymal cell proliferation and differentiation, wound healing, extracellular matrix production, immunosuppression and carcinogenesis. The formation of the receptor complex composed of 2 TGFBR1 and 2 TGFBR2 molecules symmetrically bound to the cytokine dimer results in the phosphorylation and the activation of TGFRB1 by the constitutively active TGFBR2. Activated TGFBR1 phosphorylates SMAD2 which dissociates from the receptor and interacts with SMAD4. The SMAD2-SMAD4 complex is subsequently translocated to the nucleus where it modulates the transcription of the TGF-beta-regulated genes. This constitutes the canonical SMAD-dependent TGF-beta signaling cascade. Also involved in non-canonical, SMAD-independent TGF-beta signaling pathways.
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Protein name
TGF-beta receptor type-2
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Protein short names
DNIIR; LDS1B; TBR-II; LDS2; 1110020H15RIK; TGFBR2; TBETAR-II; TAAD2; TGFR-2; MFS2; RIIC; TGFBETA-RII; TBETARII; AU042018; AAT3; FAA3; RIIDN; LDS2B
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Uniprot ID
P37173
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Gene Name
TGFBR2; hCG_1997782
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Source/Expression Host
Human Cells
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Expression Plasmid/cDNA
A DNA sequence encoding the extracellular domain (Met 1-Asp 159) of human TGF? receptor 2 (NP_003233.4) was expressed with the fused C-terminal His-tagged Fc region of human IgG1 at the C-terminus.
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Protein Species
Human
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Molecular weight
The recombinant human TGF? R2/Fc is a disulfide-linked homodimeric protein after removal of the signal peptide. The monomer consists of 383 amino acids and predicts a molecular mass of 43.4 kDa. By SDS-PAGE under reducing conditions, the apparent molecular mass of this monomer is approximately 60-65 kDa due to the glycosylation.
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Purity
> 97 % as determined by SDS-PAGE
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Activity
Measured by its ability to inhibit TGF-beta1 activity on Mv-1-lu mink lung epithelial cells.
The ED50 for this effect is typically 0.2-3.0 μg/ml in the presence of 1 ng/mL of recombinant human TGF-beta1. -
Validations
Human TGFBR2 Protein (His & Fc Tag) SDS-PAGE
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