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Product Name
TET2 antibody
- Documents
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Description
TET2 Rabbit Polyclonal antibody. Positive IP detected in mouse brain tissue. Positive WB detected in HL-60 cells, A549 cells, HEK-293 cells, human brain tissue. Observed molecular weight by Western-blot: 130kd,133kd,224kd
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Tested applications
ELISA, IP, WB
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Species reactivity
Human, Mouse; other species not tested.
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Alternative names
FLJ20032 antibody; KIAA1546 antibody; tet oncogene family member 2 antibody; TET2 antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of TET2 recombinant protein (Accession Number: BC150180). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:2000
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Validations
HL-60 cells were subjected to SDS PAGE followed by western blot with Catalog No:115953(TET2 antibody) at dilution of 1:500
IP Result of anti-TET2 (IP:Catalog No:115953, 3ug; Detection:Catalog No:115953 1:500) with mouse brain tissue lysate 4000ug.
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Background
TET2 is an enzyme which converts 5-methylcytosine (5-mC) to 5-hydroxymethylcytosine (5-hmC) in DNA and is important for normal myelopoiesis. TET2 mutations are frequently observed in myeloid malignancies, including myeloproliferative neoplasms. TET2 is broadly expressed in a variety of tissues. In healthy tissues, TET2 expression was shown to be elevated in hematopoietic cells with highest expression in granulocytes. Three isoforms of TET2 exists due to the alternative splicing. 220-250 kDa bands represent the full-length isoform; 130-133 kDa bands represent the nuclear isoforms of TET2. This antibody recognizes all three isoforms of TET2.
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References
- Zhao X, Dai J, Ma Y. Dynamics of ten-eleven translocation hydroxylase family proteins and 5-hydroxymethylcytosine in oligodendrocyte differentiation. Glia. 62(6):914-26. 2014.
- Li G, Peng J, Liu Y. Oxidized low-density lipoprotein inhibits THP-1-derived macrophage autophagy via TET2 down-regulation. Lipids. 50(2):177-83. 2015.
- Mi Y, Gao X, Dai J, Ma Y, Xu L, Jin W. A Novel Function of TET2 in CNS: Sustaining Neuronal Survival. International journal of molecular sciences. 16(9):21846-57. 2015.
- Fu HL, Ma Y, Lu LG. TET1 exerts its tumor suppressor function by interacting with p53-EZH2 pathway in gastric cancer. Journal of biomedical nanotechnology. 10(7):1217-30. 2014.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"