-
Product Name
TELO2 antibody
- Documents
-
Description
TELO2 Rabbit Polyclonal antibody. Positive IF detected in HepG2 cells. Positive IP detected in Y79 cells,. Positive WB detected in Y79 cells, A431 cells. Observed molecular weight by Western-blot: 92 kDa
-
Tested applications
ELISA, WB, IP, IF
-
Species reactivity
Human; other species not tested.
-
Alternative names
CLK2 antibody; DKFZp434A073 antibody; FLJ10924 antibody; hCLK2 antibody; KIAA0683 antibody; Protein clk 2 homolog antibody; TEL2 antibody; TELO2 antibody
-
Isotype
Rabbit IgG
-
Preparation
This antibody was obtained by immunization of TELO2 recombinant protein (Accession Number: XM_047434987). Purification method: Antigen affinity purified.
-
Clonality
Polyclonal
-
Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
-
Storage instructions
Store at -20℃. DO NOT ALIQUOT
-
Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:500-1:5000
IF: 1:10-1:100
-
Validations
Y79 cells were subjected to SDS PAGE followed by western blot with Catalog No:115941(TELO2 antibody) at dilution of 1:1500
IP Result of anti-TELO2 (IP:Catalog No:115941, 3ug; Detection:Catalog No:115941 1:1000) with Y79 cells lysate 2000ug.
IP result of TELO2 antibody (Catalog No:115941 for IP and Detection) with Y79 cell lysate.
Immunofluorescent analysis of HepG2 cells using Catalog No:115941(TELO2 Antibody) at dilution of 1:25 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
-
Background
TELO2 gene encodes a telomere length regulation protein TEL2 homolog. TELO2 may be involved in telomere length regulation and can form TTT complex with TTI1 and TTI2. TTT complex is required to stabilize protein levels of PIKK family proteins and is involved in the cellular resistance to DNA damage stresses, like ionizing radiation (IR), ultraviolet (UV) and mitomycin C (MMC). The activity of mTORC1 and mTORC2 complexes, which regulate cell growth and survival in response to nutrient and hormonal signals, can be promoted, stabilized and maintained by TELO2.
-
References
- Hurov KE, Cotta-Ramusino C, Elledge SJ. A genetic screen identifies the Triple T complex required for DNA damage signaling and ATM and ATR stability. Genes & development. 24(17):1939-50. 2010.
- Fernández-Sáiz V, Targosz BS, Lemeer S. SCFFbxo9 and CK2 direct the cellular response to growth factor withdrawal via Tel2/Tti1 degradation and promote survival in multiple myeloma. Nature cell biology. 15(1):72-81. 2013.
- Baena M, Sangüesa G, Hutter N. Fructose supplementation impairs rat liver autophagy through mTORC activation without inducing endoplasmic reticulum stress. Biochimica et biophysica acta. 1851(2):107-16. 2015.
- Kim SG, Hoffman GR, Poulogiannis G. Metabolic stress controls mTORC1 lysosomal localization and dimerization by regulating the TTT-RUVBL1/2 complex. Molecular cell. 49(1):172-85. 2013.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"