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Product Name
TELO2 antibody
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Description
TELO2 Mouse Monoclonal antibody. Positive IHC detected in human kidney tissue, human liver cancer tissue. Positive IF detected in HepG2 cells. Positive WB detected in Y79 cells, A431 cells, L02 cells. Positive IP detected in HepG2 cells. Observed molecular weight by Western-blot: 92 kDa
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Tested applications
ELISA, IF, IP, WB, IHC
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Species reactivity
Human; other species not tested.
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Alternative names
DKFZp434A073 antibody; FLJ10924 antibody; hCLK2 antibody; KIAA0683 antibody; Protein clk 2 homolog antibody; TEL2 antibody; TELO2 antibody
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Isotype
Mouse IgG1
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Preparation
This antibody was obtained by immunization of TELO2 recombinant protein (Accession Number: XM_047434987). Purification method: Protein G purified.
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Clonality
Monoclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:1000-1:10000
IP: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
Y79 cells were subjected to SDS PAGE followed by western blot with Catalog No:107619(TELO2 Antibody) at dilution of 1:2000
IP Result of anti-TELO2 (IP:Catalog No:107619, 4ug; Detection:Catalog No:107619 1:500) with HepG2 cells lysate 2900ug.
Immunofluorescent analysis of HepG2 cells, using TELO2 antibody Catalog No: at 1:25 dilution and Rhodamine-labeled goat anti-mouse IgG (red).
Immunohistochemistry of paraffin-embedded human kidney tissue slide using Catalog No:107619(TELO2 Antibody) at dilution of 1:200 (under 10x lens).
Immunohistochemistry of paraffin-embedded human kidney tissue slide using Catalog No:107619(TELO2 Antibody) at dilution of 1:200 (under 40x lens).
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Background
TELO2 gene encodes a telomere length regulation protein TEL2 homolog. TELO2 may be involved in telomere length regulation and can form TTT complex with TTI1 and TTI2. TTT complex is required to stabilize protein levels of PIKK family proteins and is involved in the cellular resistance to DNA damage stresses, like ionizing radiation (IR), ultraviolet (UV) and mitomycin C (MMC). The activity of mTORC1 and mTORC2 complexes, which regulate cell growth and survival in response to nutrient and hormonal signals, can be promoted, stabilized and maintained by TELO2.
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References
- Rao F, Cha J, Xu J. Inositol pyrophosphates mediate the DNA-PK/ATM-p53 cell death pathway by regulating CK2 phosphorylation of Tti1/Tel2. Molecular cell. 54(1):119-32. 2014.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"