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TCTN3 antibody

Cat.#: 115921

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Product Information

  • Product Name
    TCTN3 antibody
  • Documents
  • Description
    TCTN3 Rabbit Polyclonal antibody. Positive IP detected in Hela cells. Positive WB detected in Transfected HEK-293 cells. Positive IHC detected in human lung tissue. Positive IF detected in hTERT-RPE1 cells.
  • Tested applications
    ELISA, IHC, IF, IP, WB
  • Species reactivity
    Human; other species not tested.
  • Alternative names
    C10orf61 antibody; DKFZp564D116 antibody; TCTN3 antibody; TECT3 antibody; Tectonic 3 antibody; tectonic family member 3 antibody; UNQ1881/PRO4324 antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of TCTN3 recombinant protein (Accession Number: BC009494). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:500-1:5000

    IP: 1:200-1:2000

    IHC: 1:20-1:200

    IF: 1:20-1:200

  • Validations

    IP result of anti-TCTN3(Catalog No:115921 for IP and Detection).

    IP result of anti-TCTN3(Catalog No:115921 for IP and Detection).

    Immunohistochemical of paraffin-embedded human lung using Catalog No:115921(TCTN3 antibody) at dilution of 1:50 (under 40x lens)

    Immunohistochemical of paraffin-embedded human lung using Catalog No:115921(TCTN3 antibody) at dilution of 1:50 (under 40x lens)

    IF result from Dr. Corbit, Kevin. anti-TCTN3(Catalog No:115921) mark the transition zone of Human hTERT-RPE1 cells.

    IF result from Dr. Corbit, Kevin. anti-TCTN3(Catalog No:115921) mark the transition zone of Human hTERT-RPE1 cells.

    Transfected HEK-293 cells were subjected to SDS PAGE followed by western blot with Catalog No:115921(TCTN3 Antibody) at dilution of 1:1000

    Transfected HEK-293 cells were subjected to SDS PAGE followed by western blot with Catalog No:115921(TCTN3 Antibody) at dilution of 1:1000

  • Background
    TCTN3 (tectonic-3 or TECT3) is a type I membrane protein that belongs to the tectonic family, which consists of TCTN1, TCTN2 and TCTN3. Studies in mice suggest that tectonic may be involved in Hedgehog (Hh) signaling, and essential for ciliogenesis. Tectonic is component of the tectonic-like complex, a complex localized at the transition zone of primary cilia and acting as a barrier that prevents diffusion of transmembrane proteins between the cilia and plasma membranes. TCTN3 is necessary for transduction of the sonic hedgehog (SHH) signaling pathway. Orofaciodigital syndromes (OFDSs) consist of a group of heterogeneous disorders characterized by abnormalities in the oral cavity, face, and digits and associated phenotypic abnormalities. Recently, TCTN3 mutations are found as the cause of an extreme form of OFD associated with bone dysplasia, tibial defect, cystic kidneys, and brain anomalies.
  • References
    • Garcia-Gonzalo FR, Corbit KC, Sirerol-Piquer MS. A transition zone complex regulates mammalian ciliogenesis and ciliary membrane composition. Nature genetics. 43(8):776-84. 2011.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"