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Product Name
TCEB2 antibody
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Description
TCEB2 Rabbit Polyclonal antibody. Positive IHC detected in human colon cancer tissue. Positive WB detected in HeLa cells, HEK-293 cells, HL-60 cells, K-562 cells, mouse skeletal muscle tissue. Positive IP detected in HEK-293 cells. Observed molecular weight by Western-blot: 18 kDa
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Tested applications
ELISA, WB, IHC, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
ELOB antibody; Elongin 18 kDa subunit antibody; Elongin B antibody; SIII antibody; SIII p18 antibody; TCEB2 antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of TCEB2 recombinant protein (Accession Number: NM_007108). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:2000
IHC: 1:20-1:200
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:115901(TCEB2 antibody) at dilution of 1:1000
Immunohistochemical of paraffin-embedded human colon cancer using Catalog No:115901(TCEB2 antibody) at dilution of 1:100 (under 10x lens)
IP Result of anti-TCEB2 (IP:Catalog No:115901, 4ug; Detection:Catalog No:115901 1:500) with HEK-293 cells lysate 3200ug.
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Background
TCEB2 represents transcription elongation factor B (SIII), polypeptide 2 (18kDa, elongin B)
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References
- Chen J, Liu F, Li H. pVHL interacts with Ceramide kinase like (CERKL) protein and ubiquitinates it for oxygen dependent proteasomal degradation. Cellular signalling. 27(11):2314-23. 2015.
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