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Product Name
TBCC antibody
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Description
TBCC Rabbit Polyclonal antibody. Positive WB detected in HeLa cells, human brain tissue, rat liver tissue. Positive IF detected in HepG2 cells. Positive FC detected in HepG2 cells. Observed molecular weight by Western-blot: 39 kDa
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Tested applications
ELISA, WB, IF, FC
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
CFC antibody; TBCC antibody; tubulin folding cofactor C antibody; Tubulin specific chaperone C antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of TBCC recombinant protein (Accession Number: NM_003192). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IF: 1:10-1:100
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:115871(TBCC antibody) at dilution of 1:500
Immunofluorescent analysis of HepG2 cells, using TBCC antibody Catalog No:115871 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
1X10^6 HepG2 cells were stained with 0.2ug TBCC antibody (Catalog No:115871, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.
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Background
TBCC represents tubulin folding cofactor C
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"