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Product Name
TAK1 antibody
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Description
TAK1 Rabbit Polyclonal antibody. Positive WB detected in CHO cells, A2780 cells, A375 cells, A431 cells, COLO 320 cells, HeLa cells, K-562 cells, MCF7 cells, mouse heart tissue, SKOV-3 cells. Positive IHC detected in human prostate cancer tissue. Observed molecular weight by Western-blot: 64 kDa
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Tested applications
ELISA, IHC, WB
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Species reactivity
Human, hamster, Mouse; other species not tested.
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Alternative names
MAP3K7 antibody; TAK1 antibody; TGF beta activated kinase 1 antibody; TGF1a antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of TAK1 recombinant protein (Accession Number: NM_003188). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:20-1:200
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Validations
CHO cells were subjected to SDS PAGE followed by western blot with Catalog No:115842(MAP3K7 antibody) at dilution of 1:1000
Immunohistochemical of paraffin-embedded human prostate cancer using Catalog No:115842(MAP3K7 antibody) at dilution of 1:50 (under 10x lens)
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Background
MAP3K7(Mitogen-activated protein kinase kinase kinase 7) is also named as TAK1 and belongs to the MAP kinase kinase kinase subfamily.It plays an important role in the cascades of cellular responses evoked by changes in the environment.It has been linked to interleukin-1 receptor and tumor necrosis factor receptor signaling(PMID: 16186825).It has 4 isoforms (53055 kDa; 64-70 kDa and 75-80 kDa)produced by alternative splicing.
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References
- Bai C, Tang S, Bai C, Chen X. Quantitative proteomic dissection of a native 14-3-3ε interacting protein complex associated with hepatocellular carcinoma. Amino acids. 46(4):841-52. 2014.
- Zhou H, Gao S, Duan X. Inhibition of serum- and glucocorticoid-inducible kinase 1 enhances TLR-mediated inflammation and promotes endotoxin-driven organ failure. FASEB journal : official publication of the Federation of American Societies for Experimental Biology. 29(9):3737-49. 2015.
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