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Product Name
TAF1C Polyclonal Antibody
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Description
Polyclonal antibody to TAF1C
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Tested applications
WB, IHC
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Species reactivity
Human, Mouse, Rat
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Alternative names
TAF1C antibody; MGC:39976 antibody; SL1 antibody; TAFI110 antibody; TAFI95 antibody; TATA box-binding protein-associated factor RNA polymerase I subunit C antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 70-320 of human TAF1C (NP_001230085.1).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IHC 1:50 - 1:200 -
Validations
Western blot - TAF1C Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using TAF1C antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.
Immunohistochemistry - TAF1C Polyclonal Antibody
Immunohistochemistry of paraffin-embedded mouse lung using TAF1C Antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - TAF1C Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human gastric cancer using TAF1C Antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - TAF1C Polyclonal Antibody
Immunohistochemistry of paraffin-embedded mouse lung using TAF1C Antibody at dilution of 1:100 (40x lens).
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Background
Component of the transcription factor SL1/TIF-IB complex, which is involved in the assembly of the PIC (preinitiation complex) during RNA polymerase I-dependent transcription. The rate of PIC formation probably is primarily dependent on the rate of association of SL1/TIF-IB with the rDNA promoter. SL1/TIF-IB is involved in stabilization of nucleolar transcription factor 1/UBTF on rDNA. Formation of SL1/TIF-IB excludes the association of TBP with TFIID subunits. Recruits RNA polymerase I to the rRNA gene promoter via interaction with RRN3.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"