-
Product Name
Mouse CD86/B7-2 (His & Fc Tag) recombinant protein
- Documents
-
Description
Receptor involved in the costimulatory signal essential for T-lymphocyte proliferation and interleukin-2 production, by binding CD28 or CTLA-4. May play a critical role in the early events of T-cell activation and costimulation of naive T-cells, such as deciding between immunity and anergy that is made by T-cells within 24 hours after activation.
-
Protein name
T-lymphocyte activation antigen CD86
-
Protein short names
B7; MGC34413; ETC-1; CLS1; B7-2; CD28L2; TS/A-2; CD28LG2; B70; LY58; MB7; LY-58; CD86; B7.2; MB7-2; LAB72
-
Uniprot ID
Q549Q9
-
Gene Name
Cd86
-
Source/Expression Host
Human Cells
-
Expression Plasmid/cDNA
A DNA sequence encoding the extracellular domain (Met 1-Glu 245) of mouse CD86 (NP_062261.3) precursor was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus.
-
Protein Species
Mouse
-
Molecular weight
The recombinant mouse CD86/Fc is a disulfide-linked homodimer after removal of the signal peptide. The reduced monomer consists of 470 amino acids and has a predicted molecular mass of 53.4 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rm CD86/Fc monomer is approximately 70-80 kDa due to glycosylation.
-
Purity
> 95 % as determined by SDS-PAGE
-
Activity
Measured by its binding ability in a functional ELISA. Immobilized human CD28 at 20 μg/ml (100 μl/well) can bind mouse CD86 Fc Chimera with a linear ranger of 6.4-160 ng/ml.
-
Validations
Mouse CD86 / B7-2 Protein (His & Fc Tag) SDS-PAGE
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"