Human CD86/B7-2 (His Tag) recombinant protein

Human CD86/B7-2 (His Tag) recombinant protein

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Receptor involved in the costimulatory signal essential for T-lymphocyte proliferation and interleukin-2 production, by binding CD28 or CTLA-4. May play a critical role in the early events of T-cell activation and costimulation of naive T-cells, such as deciding between immunity and anergy that is made by T-cells within 24 hours after activation. Isoform 2 interferes with the formation of CD86 clusters, and thus acts as a negative regulator of T-cell activation.; (Microbial infection) Acts as a receptor for adenovirus subgroup B. {PubMed:16920215}.

T-lymphocyte activation antigen CD86

CD28LG2

P42081

CD86; CD28LG2

Human Cells

A DNA sequence encoding the extracellular domain (Met 1-His 239) of human B7-2 (NP_008820.2) was fused with a polyhistidine tag at the C-terminus.

Human

The recombinant human B7-2 consists of 228 amino acids and has a predicted molecular mass of 26.2 kDa. In SDS-PAGE, the apparent molecular mass of rhB7-2 is approximately 55-60 kDa due to glycosylation.

> 97 % as determined by SDS-PAGE

Measured by its binding ability in a functional ELISA. Immobilized human CD86 at 20 μg/ml (100 μl/well) can bind human CD28 with a linear ranger of 32-800 ng/ml.