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Product Name
SYT1 Polyclonal Antibody
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Description
Polyclonal antibody to SYT1
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Tested applications
WB, IHC
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Species reactivity
Human, Mouse, Rat
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Alternative names
SYT1 antibody; P65 antibody; SVP65 antibody; SYT antibody; synaptotagmin-1 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-422 of human SYT1 (NP_001129278.1).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IHC 1:50 - 1:200 -
Validations
Western blot - SYT1 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using SYT1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .
Immunohistochemistry - SYT1 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded rat kidney using SYT1 antibody at dilution of 1:200 (40x lens).
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Background
May have a regulatory role in the membrane interactions during trafficking of synaptic vesicles at the active zone of the synapse. It binds acidic phospholipids with a specificity that requires the presence of both an acidic head group and a diacyl backbone. A Ca(2+)-dependent interaction between synaptotagmin and putative receptors for activated protein kinase C has also been reported. It can bind to at least three additional proteins in a Ca(2+)-independent manner; these are neurexins, syntaxin and AP2. Plays a role in dendrite formation by melanocytes.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"