-
Product Name
SYNM antibody
- Documents
-
Description
SYNM Rabbit Polyclonal antibody. Positive IF detected in HeLa cells. Positive IHC detected in human skeletal muscle tissue, human bladder tissue, human heart tissue. Positive WB detected in Y79 cells, human skeletal muscle tissue, Jurkat cells. Observed molecular weight by Western-blot: 170-180kd
-
Tested applications
ELISA, WB, IHC, IF
-
Species reactivity
Human; other species not tested.
-
Alternative names
Desmuslin antibody; DMN antibody; KIAA0353 antibody; SYN antibody; Synemin antibody; SYNM antibody
-
Isotype
Rabbit IgG
-
Preparation
This antibody was obtained by immunization of Peptide (Accession Number: NM_015286). Purification method: Antigen affinity purified.
-
Clonality
Polyclonal
-
Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
-
Storage instructions
Store at -20℃. DO NOT ALIQUOT
-
Applications
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:10-1:100
-
Validations
Y79 cells were subjected to SDS PAGE followed by western blot with Catalog No:115786(SYNM antibody) at dilution of 1:1000
Immunohistochemical of paraffin-embedded human skeletal muscle using Catalog No:115786(SYNM antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human skeletal muscle using Catalog No:115786(SYNM antibody) at dilution of 1:50 (under 40x lens)
Immunofluorescent analysis of HeLa cells using Catalog No:115786(SYNM Antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG
-
Background
SYNM, also named as DMN, KIAA0353 and SYN, belongs to the intermediate filament family. SYNM is a Type-VI intermediate filament (IF) which plays an important cytoskeletal role within the muscle cell cytoskeleton. It forms heteropolymeric IFs with desmin and/or vimentin, and via its interaction with cytoskeletal proteins alpha-dystrobrevin, dystrophin, talin-1, utrophin and vinculin, is able to link these heteropolymeric IFs to adherens-type junctions, such as to the costameres, neuromuscular junctions, and myotendinous junctions within striated muscle cells. The antibody can’t recognize isofor2 and 3 of SYNM.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"