Product Name

SYK antibody

Documents

Description

SYK Rabbit Polyclonal antibody. Positive WB detected in HEK-293 cells. Positive IHC detected in human spleen tissue. Observed molecular weight by Western-blot: 75 kDa

Tested applications

ELISA, WB, IHC

Species reactivity

Human; other species not tested.

Alternative names

DKFZp313N1010 antibody; FLJ25043 antibody; FLJ37489 antibody; spleen tyrosine kinase antibody; SYK antibody; Tyrosine protein kinase SYK antibody

Isotype

Rabbit IgG

Preparation

This antibody was obtained by immunization of SYK recombinant protein (Accession Number: XM_005252147). Purification method: Antigen affinity purified.

Clonality

Polyclonal

Formulation

PBS with 0.02% sodium azide and 50% glycerol pH 7.3.

Storage instructions

Store at -20℃. DO NOT ALIQUOT

Applications

Recommended Dilution:

WB: 1:500-1:5000

IHC: 1:20-1:200

Validations

HEK-293 cells were subjected to SDS PAGE followed by western blot with Catalog No:115761(SYK antibody) at dilution of 1:1000

Immunohistochemistry of paraffin-embedded human spleen tissue slide using Catalog No:115761(SYK Antibody) at dilution of 1:50 (under 10x lens)

Immunohistochemistry of paraffin-embedded human spleen tissue slide using Catalog No:115761(SYK Antibody) at dilution of 1:50 (under 40x lens)

Background

SYK(spleen tyrosine kinase) is a key regulator of signal transduction events,apoptosis and orderly cell cycle progression in B-lineage lymphoid cells. Although SYK has not been linked to a human disease, defective expression of the closely related T-cell tyrosine kinase ZAP-70 has been associated with severe combined immunodeficiency(PMID:11494125). This protein has 2 isoforms produced by alternative splicing.

References

Yin H, Zhou H, Kang Y. Syk negatively regulates TLR4-mediated IFNβ and IL-10 production and promotes inflammatory responses in dendritic cells. Biochimica et biophysica acta. 1860(3):588-98. 2016.

SYK antibody

Product Information

Related Products / Services