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SURVIVIN antibody

Cat.#: 115749

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Product Information

  • Product Name
    SURVIVIN antibody
  • Documents
  • Description
    SURVIVIN Rabbit Polyclonal antibody. Positive IF detected in HeLa cells. Positive IHC detected in human breast cancer tissue, human colon cancer tissue, human gliomas tissue, human lung cancer tissue, human pancreas cancer tissue, rat brain tissue. Positive WB detected in PC-3 cells, A431 cells, A549 cells, Jurkat cells, MCF7 cells. Positive IP detected in Jurkat cells. Observed molecular weight by Western-blot: 16-18 kDa
  • Tested applications
    ELISA, WB, IF, IP, IHC
  • Species reactivity
    Human, Rat; other species not tested.
  • Alternative names
    API4 antibody; Apoptosis inhibitor 4 antibody; Apoptosis inhibitor survivin antibody; BIRC5 antibody; EPR 1 antibody; IAP4 antibody; SURVIVIN antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of SURVIVIN recombinant protein (Accession Number: NM_001168). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:200-1:2000

    IP: 1:200-1:1000

    IHC: 1:50-1:500

    IF: 1:10-1:100

  • Validations

    PC-3 cells were subjected to SDS PAGE followed by western blot with Catalog No:115749(SURVIVIN antibody) at dilution of 1:1000

    PC-3 cells were subjected to SDS PAGE followed by western blot with Catalog No:115749(SURVIVIN antibody) at dilution of 1:1000

    Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using Catalog No:115749(SURVIVIN Antibody) at dilution of 1:200 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

    Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using Catalog No:115749(SURVIVIN Antibody) at dilution of 1:200 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

    Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using Catalog No:115749(SURVIVIN Antibody) at dilution of 1:200 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

    Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using Catalog No:115749(SURVIVIN Antibody) at dilution of 1:200 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

    IP Result of anti-SURVIVIN (IP:Catalog No:115749, 4ug; Detection:Catalog No:115749 1:300) with Jurkat cells lysate 2120ug.

    IP Result of anti-SURVIVIN (IP:Catalog No:115749, 4ug; Detection:Catalog No:115749 1:300) with Jurkat cells lysate 2120ug.

    Immunofluorescent analysis of HeLa cells using Catalog No:115749(SURVIVIN Antibody) at dilution of 1:25 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

    Immunofluorescent analysis of HeLa cells using Catalog No:115749(SURVIVIN Antibody) at dilution of 1:25 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

  • Background
    Survivin, also called BIRC5, is a unique member of the inhibitor of apoptosis (IAP) protein family. Survivin is a 16 kDa anti-apoptotic protein highly expressed during fetal development and cancer cell malignancy, but is completely absent in terminally differentiated cells. The differential expression of survivin in cancer versus normal tissues makes it a useful tool in cancer diagnosis and a promising therapeutic target. Survivin expression is also highly regulated by the cell cycle and is only expressed in the G2-M phase. It is known that survivin localizes to the mitotic spindle by interaction with tubulin during mitosis and may play a contributing role in regulating mitosis. Disruption of survivin-microtubule interactions results in loss of survivin's anti-apoptosis function and increased caspase-3 activity, a mechanism involved in cell death, during mitosis. It also is a direct target gene of the Wnt pathway and is upregulated by beta-catenin.
  • References
    • Wu J, Chen W, Yin Y, Zheng Z, Zou G. Probing the cell death signaling pathway of HepG2 cell line induced by copper-1,10-phenanthroline complex. Biometals : an international journal on the role of metal ions in biology, biochemistry, and medicine. 27(3):445-58. 2014.
    • Yu B, Tang C, Yin C. Enhanced antitumor efficacy of folate modified amphiphilic nanoparticles through co-delivery of chemotherapeutic drugs and genes. Biomaterials. 35(24):6369-78. 2014.
    • Zhang MZ, Li C, Fang BY. High transfection efficiency of quantum dot-antisense oligonucleotide nanoparticles in cancer cells through dual-receptor synergistic targeting. Nanotechnology. 25(25):255102. 2014.
    • Li X, Liu X, Xu Y. KLF5 promotes hypoxia-induced survival and inhibits apoptosis in non-small cell lung cancer cells via HIF-1α. International journal of oncology. 45(4):1507-14. 2014.
    • Li X, Liu X, Xu Y, He Y, Liu J, Xie M. Expression profile of apoptotic and proliferative proteins in hypoxic HUVEC treated with statins. International journal of oncology. 46(2):677-84. 2015.
    • Cotroneo E, Ashek A, Wang L. Iron homeostasis and pulmonary hypertension: iron deficiency leads to pulmonary vascular remodeling in the rat. Circulation research. 116(10):1680-90. 2015.
    • Huang W, Liu J, Feng X. DLC-1 induces mitochondrial apoptosis and epithelial mesenchymal transition arrest in nasopharyngeal carcinoma by targeting EGFR/Akt/NF-κB pathway. Medical oncology (Northwood, London, England). 32(4):115. 2015.
    • Zhao X, Fang Y, Yang Y. Elaiophylin, a novel autophagy inhibitor, exerts antitumor activity as a single agent in ovarian cancer cells. Autophagy. 11(10):1849-63. 2015.

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