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Product Name
SUOX antibody
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Description
SUOX Rabbit Polyclonal antibody. Positive WB detected in human liver tissue, L02 cells, mouse liver tissue. Positive FC detected in HeLa cells. Positive IF detected in Hela cells. Positive IHC detected in human liver tissue. Observed molecular weight by Western-blot: 52 kDa, 60 kDa
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Tested applications
ELISA, WB, IHC, IF, FC
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
sulfite oxidase antibody; Sulfite oxidase antibody; mitOChondrial antibody; SUOX antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of SUOX recombinant protein (Accession Number: NM_000456). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
human liver tissue were subjected to SDS PAGE followed by western blot with Catalog No:115840(SUOX antibody) at dilution of 1:1000
Immunohistochemical of paraffin-embedded human liver using Catalog No:115840(SUOX antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human liver using Catalog No:115840(SUOX antibody) at dilution of 1:100 (under 40x lens)
Immunofluorescent analysis of Hela cells, using SUOX antibody Catalog No:115840 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
1X10^6 HeLa cells were stained with 0.2ug SUOX antibody (Catalog No:115840, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.
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Background
SUOX(sulfite oxidase) catalyzes the oxidation of sulfite to sulfate, the terminal reaction in the oxidative degradation pathway of the sulfur-containing amino acids cysteine and methionine. The enzyme is a dimer of identical subunits and is located in the intermembrane space of mitochondria(PMID:9600976). Defects in SUOX are the cause of isolated sulfite oxidase deficiency (ISOD).
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References
- Zhao X, Cong X, Zheng L, Xu L, Yin L, Peng J. Dioscin, a natural steroid saponin, shows remarkable protective effect against acetaminophen-induced liver damage in vitro and in vivo. Toxicology letters. 214(1):69-80. 2012.
- Roman HB, Hirschberger LL, Krijt J, Valli A, Kožich V, Stipanuk MH. The cysteine dioxgenase knockout mouse: altered cysteine metabolism in nonhepatic tissues leads to excess H2S/HS(-) production and evidence of pancreatic and lung toxicity. Antioxidants & redox signaling. 19(12):1321-36. 2013.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"