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Product Name
SULT1E1 antibody
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Description
SULT1E1 Rabbit Polyclonal antibody. Positive IP detected in mouse liver tissue. Positive WB detected in human lung tissue, human adrenal gland tissue, human kidney tissue, human liver tissue, mouse kidney tissue. Observed molecular weight by Western-blot: 32-35 kDa
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Tested applications
ELISA, WB, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
EST antibody; EST 1 antibody; Estrogen sulfotransferase antibody; ST1E1 antibody; STE antibody; Sulfotransferase 1E1 antibody; SULT1E1 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of SULT1E1 recombinant protein (Accession Number: XM_047416101). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:1000
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Validations
human lung tissue were subjected to SDS PAGE followed by western blot with Catalog No:115830(SULT1E1 antibody) at dilution of 1:500
IP Result of anti-SULT1E1 (IP:Catalog No:115830, 4ug; Detection:Catalog No:115830 1:400) with mouse liver tissue lysate 4000ug.
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Background
SULT1E1, also known as estrogen sulfotransferase, catalyzes the sulfation of endogenous estrogens as well as xenobiotic estrogen-like chemicals, converting them into the inactive form. SULT1E1 is a 33-35 kDa cytosolic protein that has been detected in human hepatic and nonhepatic tissues (17035602). SULT1E1 is expressed in breast and endometrial tissues, and some studies have investigated its implication in tumor development. Higher expression of SULT1E1 was observed in breast cancer tissues compared with normal breast tissues (22380844). This antibody detected a major band around 35 kD in lysates of mouse kidney, human liver and so on.
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References
- Xu Y, Yang X, Wang Z. Estrogen sulfotransferase (SULT1E1) regulates inflammatory response and lipid metabolism of human endothelial cells via PPARγ. Molecular and cellular endocrinology. 369(1-2):140-9. 2013.
- Guo Y, Hu B, Huang H. Estrogen Sulfotransferase Is an Oxidative Stress-responsive Gene That Gender-specifically Affects Liver Ischemia/Reperfusion Injury. The Journal of biological chemistry. 290(23):14754-64. 2015.
- Chai X, Guo Y, Jiang M. Oestrogen sulfotransferase ablation sensitizes mice to sepsis. Nature communications. 6:7979. 2015.
- Li Y, Xu Y, Li X, Qin Y, Hu R. Effects of PPAR-α agonist and IGF-1 on estrogen sulfotransferase in human vascular endothelial and smooth muscle cells. Molecular medicine reports. 8(1):133-9. 2013.
- Xu Y, Liu X, Guo F. Effect of estrogen sulfation by SULT1E1 and PAPSS on the development of estrogen-dependent cancers. Cancer science. 103(6):1000-9. 2012.
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