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Product Name
SULT1A1 antibody
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Description
SULT1A1 Rabbit Polyclonal antibody. Positive IP detected in mouse liver tissue. Positive WB detected in mouse liver tissue, Jurkat cells, rat liver tissue. Positive IF detected in HepG2 cells. Positive FC detected in HepG2 cells. Observed molecular weight by Western-blot: 34kd
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Tested applications
ELISA, WB, IF, IP, FC
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
Aryl sulfotransferase 1 antibody; HAST1/HAST2 antibody; P PST antibody; P PST 1 antibody; Phenol sulfotransferase 1 antibody; PST antibody; ST1A1 antibody; ST1A3 antibody; STP antibody; STP1 antibody; Sulfotransferase 1A1 antibody; SULT1A1 antibody; Ts PST antibody; TSPST1 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of SULT1A1 recombinant protein (Accession Number: XM_017023611). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:500-1:5000
IF: 1:20-1:200
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Validations
mouse liver tissue were subjected to SDS PAGE followed by western blot with Catalog No:115825(SULT1A1 antibody) at dilution of 1:1000
IP Result of anti-SULT1A1 (IP:Catalog No:115825, 3ug; Detection:Catalog No:115825 1:1000) with mouse liver tissue lysate 12500ug.
Immunofluorescent analysis of HepG2 cells, using SULT1A1 antibody Catalog No:115825 at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
1X10^6 HepG2 cells were stained with 0.2ug SULT1A1 antibody (Catalog No:115825, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.
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Background
SULT1A1, also named as ST1A1, P-PST 1, ST1A3, Ts-PST, P-PST 1, STP and STP1, belongs to the sulfotransferase 1 family. It catalyzes the sulfate conjugation of catecholamines, phenolic drugs and neurotransmitters. SULT1A1 is also responsible for the sulfation and activation of minoxidil. It mediates the metabolic activation of carcinogenic N-hydroxyarylamines to DNA binding products and could so participate as modulating factor of cancer risk. SULT1A1 is one of two phenol sulfotransferases with thermostable enzyme activity.
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References
- Shu XH, Li H, Sun Z. Identification of metabolic pattern and bioactive form of resveratrol in human medulloblastoma cells. Biochemical pharmacology. 79(10):1516-25. 2010.
- Shu XH, Li H, Sun XX. Metabolic patterns and biotransformation activities of resveratrol in human glioblastoma cells: relevance with therapeutic efficacies. PloS one. 6(11):e27484. 2011.
- Sun Z, Li H, Shu XH. Distinct sulfonation activities in resveratrol-sensitive and resveratrol-insensitive human glioblastoma cells. The FEBS journal. 279(13):2381-92. 2012.
- Sun Z, Shi S, Li H. Evaluation of resveratrol sensitivities and metabolic patterns in human and rat glioblastoma cells. Cancer chemotherapy and pharmacology. 72(5):965-73. 2013.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"