STXBP5 antibody

Cat.#: 115813

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Product Information

  • Product Name
    STXBP5 antibody
  • Documents
  • Description
    STXBP5 Rabbit Polyclonal antibody. Positive WB detected in rat brain tissue. Positive IP detected in rat brain tissue. Positive IF detected in SH-SY5Y cells. Observed molecular weight by Western-blot: 130 kDa
  • Tested applications
    ELISA, WB, IP, IF
  • Species reactivity
    Human, Rat; other species not tested.
  • Alternative names
    FLJ30922 antibody; LGL3 antibody; LLGL3 antibody; Nbla04300 antibody; STXBP5 antibody; Syntaxin binding protein 5 antibody; Tomosyn 1 antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of STXBP5 recombinant protein (Accession Number: NM_139244). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:200-1:1000

    IP: 1:200-1:1000

    IF: 1:10-1:100

  • Validations

    rat brain tissue were subjected to SDS PAGE followed by western blot with Catalog No:115813(STXBP5 Antibody) at dilution of 1:300

    rat brain tissue were subjected to SDS PAGE followed by western blot with Catalog No:115813(STXBP5 Antibody) at dilution of 1:300

    IP Result of anti-STXBP5 (IP:Catalog No:115813, 5ug; Detection:Catalog No:115813 1:300) with rat brain tissue lysate 4000ug.

    IP Result of anti-STXBP5 (IP:Catalog No:115813, 5ug; Detection:Catalog No:115813 1:300) with rat brain tissue lysate 4000ug.

    Immunofluorescent analysis of SH-SY5Y cells using Catalog No:115813(STXBP5 Antibody) at dilution of 1:25 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

    Immunofluorescent analysis of SH-SY5Y cells using Catalog No:115813(STXBP5 Antibody) at dilution of 1:25 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

  • Background
    Syntaxin-binding protein 5 (STXBP5, also known as tomosyn) is a cytosolic syntaxin-binding protein that can displace MUNC18 from syntaxin-1. It is a soluble R-SNARE protein that sequesters target SNAREs through the C-terminal VAMP-like domain. STXBP5 plays a regulatory role in calcium-dependent exocytosis and neurotransmitter release.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"