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Product Name
STS antibody
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Description
STS Rabbit Polyclonal antibody. Positive WB detected in MCF7 cells, human placenta tissue. Positive IHC detected in human placenta tissue, human hysteromyoma tissue, human kidney tissue, human lung tissue, human ovary tissue, human paracancerous tissue of hysteromyoma, human skin tissue. Observed molecular weight by Western-blot: 63kd-65kd
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Tested applications
ELISA, WB, IHC
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
ARSC antibody; ARSC1 antibody; Arylsulfatase C antibody; ASC antibody; SSDD antibody; Steroid sulfatase antibody; Steryl sulfatase antibody; Steryl sulfate sulfohydrolase antibody; STS antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of STS recombinant protein (Accession Number: XM_047442107). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:20-1:200
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Validations
MCF7 cells were subjected to SDS PAGE followed by western blot with Catalog No:115738(STS antibody) at dilution of 1:500
Immunohistochemical of paraffin-embedded human placenta using Catalog No:115738(STS antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human placenta using Catalog No:115738(STS antibody) at dilution of 1:100 (under 40x lens)
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Background
STS(Steroid sulfatase) is also named as ASC(arylsulfatase C), ARSC1 and belongs to the sulfatase family. It is expressed in human liver and is responsible for the hydrolysis of many estrogen and hydroxysteroid sulfates, including dehydroepiandrosterone (DHEA)-sulfate and β-estradiol(E2)-3-sulfate(PMID:19589875). This enzyme has also been documented to occur in many other tissues, including skin, lung, ovary, and adrenal gland. The estimated molecular mass of expressed human STS is 63-85 kDa which may represent different levels of glycosylation in the different tissues, as STS is known to be a glycoprotein and the full length protein has a signal peptide with 21 amino acids(PMID:17604157).
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References
- Li J, Peng X, Zeng X. Estrogen Secreted by Mesenchymal Stem Cells Necessarily Determines Their Feasibility of Therapeutical Application. Scientific reports. 5:15286. 2015.
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