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Product Name
STOM antibody
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Description
STOM Rabbit Polyclonal antibody. Positive IF detected in A549 cells. Positive IHC detected in human lung cancer tissue. Positive IP detected in HeLa cells. Positive WB detected in human heart tissue, HeLa cells, human liver tissue, human lung tissue, K-562 cells. Observed molecular weight by Western-blot: 32 kDa
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Tested applications
ELISA, WB, IHC, IP, IF
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Species reactivity
Human; other species not tested.
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Alternative names
BND7 antibody; EPB7 antibody; EPB72 antibody; Protein 7.2b antibody; STOM antibody; stomatin antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of STOM recombinant protein (Accession Number: NM_004099). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:1000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
Immunofluorescent analysis of A549 cells using Catalog No:115729(STOM Antibody) at dilution of 1:25 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
human heart tissue were subjected to SDS PAGE followed by western blot with Catalog No:115729(STOM antibody) at dilution of 1:500
Immunohistochemical of paraffin-embedded human lung cancer using Catalog No:115729(STOM antibody) at dilution of 1:100 (under 10x lens)
IP Result of anti-STOM (IP:Catalog No:115729, 3ug; Detection:Catalog No:115729 1:300) with HeLa cells lysate 2800ug.
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Background
STOM, also named as BND7, EPB72, Stomatin and Protein 7.2b, belongs to the band 7/mec-2 family. It is thought to regulate cation conductance. STOM may regulate ACCN1 and ACCN3 gating. STOM is a membrane protein involved in regulation of monovalent cation transport through lipid membranes. Interestingly, STOM (which has a structure similar to caveolin) is a major lipid-raft component of erythrocytes. It may have a role in membrane microdomains modulation leading to membrane budding and MPs release.(PMID:19138258)
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References
- Rubin O, Crettaz D, Canellini G, Tissot JD, Lion N. Microparticles in stored red blood cells: an approach using flow cytometry and proteomic tools. Vox sanguinis. 95(4):288-97. 2008.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"