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STAT5A antibody

Cat.#: 115691

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Product Information

  • Product Name
    STAT5A antibody
  • Documents
  • Description
    STAT5A Rabbit Polyclonal antibody. Positive IHC detected in human breast cancer tissue, human cervical cancer tissue. Positive FC detected in MCF-7 cells. Positive IP detected in 3T3-L1 cells. Positive WB detected in K-562 cells, 3T3-L1 cells, DU 145 cells. Observed molecular weight by Western-blot: 90-95 kDa,50-55 kDa
  • Tested applications
    ELISA, WB, IHC, FC, IP
  • Species reactivity
    Human,Mouse,Rat; other species not tested.
  • Alternative names
    MGF antibody; STAT5 antibody; STAT5A antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of STAT5A recombinant protein (Accession Number: NM_003152). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:500-1:5000

    IP: 1:500-1:5000

    IHC: 1:20-1:200

  • Validations

    K-562 cells were subjected to SDS PAGE followed by western blot with Catalog No:115691(STAT5A antibody) at dilution of 1:1000

    K-562 cells were subjected to SDS PAGE followed by western blot with Catalog No:115691(STAT5A antibody) at dilution of 1:1000

    Immunohistochemical of paraffin-embedded human breast cancer using Catalog No:115691(STAT5A antibody) at dilution of 1:100 (under 10x lens)

    Immunohistochemical of paraffin-embedded human breast cancer using Catalog No:115691(STAT5A antibody) at dilution of 1:100 (under 10x lens)

    IP Result of anti-STAT5A (IP:Catalog No:115691, 4ug; Detection:Catalog No:115691 1:1000) with 3T3-L1 cells lysate 3200ug.

    IP Result of anti-STAT5A (IP:Catalog No:115691, 4ug; Detection:Catalog No:115691 1:1000) with 3T3-L1 cells lysate 3200ug.

    1X10^6 MCF-7 cells were stained with 0.2ug STAT5A antibody (Catalog No:115691, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

    1X10^6 MCF-7 cells were stained with 0.2ug STAT5A antibody (Catalog No:115691, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

  • Background
    STAT5A, also named as STAT5, belongs to the transcription factor STAT family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. STAT5A is activated by, and mediates the responses of many cell ligands, such as IL2, IL3, IL7 GM-CSF, erythropoietin, thrombopoietin, and different growth hormones. Activation of STAT5A in myeloma and lymphoma associated with a TEL/JAK2 gene fusion is independent of cell stimulus and has been shown to be essential for the tumorigenesis. The mouse counterpart of human STAT5A is found to induce the expression of BCL2L1/BCL-X(L), which suggests the antiapoptotic function of STAT5A in cells. This antibody is a rabbit polyclonal antibody raised against residues near the C terminus of human STAT5A.
  • References
    • Dong Z, Lu X, Yang Y. IL-27 alleviates the bleomycin-induced pulmonary fibrosis by regulating the Th17 cell differentiation. BMC pulmonary medicine. 15:13. 2015.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"