-
Product Name
ST3GAL4 Polyclonal Antibody
- Documents
-
Description
Polyclonal antibody to ST3GAL4
-
Tested applications
WB, IF
-
Species reactivity
Human, Rat
-
Alternative names
ST3GAL4 antibody; CGS23 antibody; NANTA3 antibody; SAT3 antibody; SIAT4 antibody; SIAT4C antibody; ST-4 antibody; ST3GalA.2 antibody; ST3GalIV antibody; STZ antibody; gal-NAc6S antibody; ST3 beta-galactoside alpha-2,3-sialyltransferase 4 antibody
-
Isotype
Rabbit IgG
-
Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 27-267 of human ST3GAL4 (NP_006269.1).
-
Clonality
Polyclonal
-
Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
-
Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
-
Applications
WB 1:500 - 1:2000
IF 1:10 - 1:100 -
Validations
Western blot - ST3GAL4 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using ST3GAL4 antibody at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit ._Exposure time: 60s.
Immunofluorescence - ST3GAL4 Polyclonal Antibody
Immunofluorescence analysis of HeLa cells using ST3GAL4 antibody . Blue: DAPI for nuclear staining.
-
Background
Catalyzes the formation of the NeuAc-alpha-2,3-Gal-beta-1,4-GlcNAc-, and NeuAc-alpha-2,3-Gal-beta-1,3-GlcNAc- sequences found in terminal carbohydrate groups of glycoproteins and glycolipids. It may be involved in the biosynthesis of the sialyl Lewis X determinant.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"