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Product Name
ST3GAL4 Polyclonal Antibody
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Description
Polyclonal antibody to ST3GAL4
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Tested applications
WB, IHC
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Species reactivity
Mouse, Rat
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Alternative names
ST3GAL4 antibody; CGS23 antibody; NANTA3 antibody; SAT3 antibody; SIAT4 antibody; SIAT4C antibody; ST-4 antibody; ST3GalA.2 antibody; ST3GalIV antibody; STZ antibody; gal-NAc6S antibody; ST3 beta-galactoside alpha-2,3-sialyltransferase 4 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 27-267 of human ST3GAL4 (NP_006269.1).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IHC 1:50 - 1:200 -
Validations
Western blot - ST3GAL4 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using ST3GAL4 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit .Exposure time: 60s.
Immunohistochemistry - ST3GAL4 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded rat kidney using ST3GAL4 antibody at dilution of 1:200 (40x lens).
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Background
Catalyzes the formation of the NeuAc-alpha-2,3-Gal-beta-1,4-GlcNAc-, and NeuAc-alpha-2,3-Gal-beta-1,3-GlcNAc- sequences found in terminal carbohydrate groups of glycoproteins and glycolipids. It may be involved in the biosynthesis of the sialyl Lewis X determinant.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"