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Product Name
SSB antibody
- Documents
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Description
SSB Rabbit Polyclonal antibody. Positive IHC detected in human colon cancer tissue. Positive IF detected in HeLa cells. Positive WB detected in Jurkat cells, HEK-293 cells, HeLa cells. Positive IP detected in Jurkat cells. Observed molecular weight by Western-blot: 48 kDa
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Tested applications
ELISA, IHC, WB, IF, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
La autoantigen antibody; La ribonucleoprotein antibody; LARP3 antibody; Lupus La protein antibody; SS B antibody; SSB antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of SSB recombinant protein (Accession Number: NM_003142). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:1000
IP: 1:200-1:1000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
Jurkat cells were subjected to SDS PAGE followed by western blot with Catalog No:115605(SSB Antibody) at dilution of 1:300
Immunohistochemical of paraffin-embedded human colon cancer using Catalog No:115605(SSB antibody) at dilution of 1:50 (under 10x lens)
IP Result of anti-SSB (IP:Catalog No:115605, 4ug; Detection:Catalog No:115605 1:300) with Jurkat cells lysate 3600ug.
Immunofluorescent analysis of HeLa cells using Catalog No:115605(SSB Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
SSB represents Sjogren syndrome antigen B (autoantigen La)
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References
- Hao R, He J, Liu X. Inhibition of hepatitis B virus gene expression and replication by hepatocyte nuclear factor 6. Journal of virology. 89(8):4345-55. 2015.
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