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Product Name
SRY antibody
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Description
SRY Rabbit Polyclonal antibody. Positive WB detected in human testis tissue. Positive IHC detected in human kidney tissue, human spleen tissue, human testis tissue. Observed molecular weight by Western-blot: 30kd
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Tested applications
ELISA, WB, IHC
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Species reactivity
Human, Mouse; other species not tested.
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Alternative names
sex determining region Y antibody; SRY antibody; TDF antibody; TDY antibody; Testis determining factor antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of SRY recombinant protein (Accession Number: NM_003140). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IHC: 1:20-1:200
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Validations
human testis tissue were subjected to SDS PAGE followed by western blot with Catalog No:115603(SRY antibody) at dilution of 1:500
Immunohistochemical of paraffin-embedded human kidney using Catalog No:115603(SRY antibody) at dilution of 1:100 (under 10x lens)
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Background
SRY (sex-determining region Y protein) is a tran-scriptional activator required for male sex determination in mammals. This protein, also referred to as testis-determining factor (TDF), is an HMG box protein that initiates the formation of testis from undifferentiated gonad. The DNA-binding activity of SRY is required for normal testis formation. This DNA-binding activity is thought to be regulated by PKA, which phosphorylates SRY in vivo. Mutations in SRY have been associated with 46,XY gonadal dysgenesis, in which the gonads fail to develop in XY phenotypic females.
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