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Product Name
SRSF1 Polyclonal Antibody
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Description
Polyclonal antibody to SRSF1
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Tested applications
WB, IHC, IF
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Species reactivity
Human, Mouse, Rat
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Alternative names
SRSF1 antibody; ASF antibody; SF2 antibody; SF2p33 antibody; SFRS1 antibody; SRp30a antibody; serine/arginine-rich splicing factor 1 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-248 of human SRSF1 (NP_008855.1).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200 -
Validations
Western blot - SRSF1 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using SRSF1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.
Immunohistochemistry - SRSF1 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded rat lung using SRSF1 Antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - SRSF1 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human lung cancer using SRSF1 Antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - SRSF1 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded mouse esophagus using SRSF1 Antibody at dilution of 1:100 (40x lens).
Immunofluorescence - SRSF1 Polyclonal Antibody
Immunofluorescence analysis of U2OS cells using SRSF1 antibody . Blue: DAPI for nuclear staining.
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Background
Plays a role in preventing exon skipping, ensuring the accuracy of splicing and regulating alternative splicing. Interacts with other spliceosomal components, via the RS domains, to form a bridge between the 5'- and 3'-splice site binding components, U1 snRNP and U2AF. Can stimulate binding of U1 snRNP to a 5'-splice site-containing pre-mRNA. Binds to purine-rich RNA sequences, either the octamer, 5'-RGAAGAAC-3' (r=A or G) or the decamers, AGGACAGAGC/AGGACGAAGC. Binds preferentially to the 5'-CGAGGCG-3' motif in vitro. Three copies of the octamer constitute a powerful splicing enhancer in vitro, the ASF/SF2 splicing enhancer (ASE) which can specifically activate ASE-dependent splicing. Isoform ASF-2 and isoform ASF-3 act as splicing repressors. May function as export adapter involved in mRNA nuclear export through the TAP/NXF1 pathway.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"