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Product Name
SRP54 antibody
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Description
SRP54 Rabbit Polyclonal antibody. Positive WB detected in human placenta tissue, NIH/3T3 cells. Positive IP detected in human placenta tissue. Positive IHC detected in human breast cancer tissue. Observed molecular weight by Western-blot: 54kd
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Tested applications
ELISA, WB, IHC, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
SRP54 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of SRP54 recombinant protein (Accession Number: XM_011537106). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:2000
IHC: 1:20-1:200
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Validations
human placenta tissue were subjected to SDS PAGE followed by western blot with Catalog No:115592(SRP54 antibody) at dilution of 1:500
IP Result of anti-SRP54 (IP:Catalog No:115592, 4ug; Detection:Catalog No:115592 1:500) with human placenta tissue lysate 4000ug.
Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using Catalog No:115592(SRP54 Antibody) at dilution of 1:50 (under 40x lens)
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Background
The signal recognition particle (SRP) is a ribonucleoprotein complex that mediates the targeting of proteins to the endoplasmic reticulum (ER). The complex consists of a 7S (or 7SL) RNA and 6 different proteins, and signal recognition particle 54 (SRP54) is one of them. SRP54 binds to the signal sequence of presecretory protein as they emerge from the translating ribosomes, and then transfers them to translocating chain-associating membrane protein (TRAM).
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References
- Ren YF, Li G, Xue YF. Decreased dicer expression enhances SRP-mediated protein targeting. PloS one. 8(2):e56950. 2013.
- Ueda M, Li S, Itoh M. Polyglutamine expansion disturbs the endoplasmic reticulum formation, leading to caspase-7 activation through Bax. Biochemical and biophysical research communications. 443(4):1232-8. 2014.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"