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Product Name
SPRY4 antibody
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Description
SPRY4 Rabbit Polyclonal antibody. Positive WB detected in A375 cells. Positive IHC detected in human cervical cancer tissue, human heart tissue. Observed molecular weight by Western-blot: 35-38 kDa
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Tested applications
ELISA, WB, IHC
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Species reactivity
Human; other species not tested.
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Alternative names
Protein sprouty homolog 4 antibody; sprouty homolog 4 (Drosophila) antibody; Spry 4 antibody; SPRY4 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of SPRY4 recombinant protein (Accession Number: NM_001293289). Purification method: Antigen Affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:20-1:200
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Validations
A375 cells were subjected to SDS PAGE followed by western blot with Catalog No:115650(SPRY4 Antibody) at dilution of 1:1000
Immunohistochemistry of paraffin-embedded human cervical cancer tissue slide using Catalog No:115650(SPRY4 Antibody) at dilution of 1:50 (under 40x lens)
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Background
SPRY4, also named as Protein sprouty homolog 4, is a 299 amino acid protein, which contains one SPR (sprouty) domain and belongs to the sprouty family. SPRY4 Suppresses the insulin receptor and EGFR-transduced MAPK signaling pathway, but does not inhibit MAPK activation by a constitutively active mutant Ras. Probably impairs the formation of GTP-Ras. SPRY4 has a cellular function to regulate the actin cytoskeleton, independent of its inhibitory activity on the Ras/MAP kinase signalling. SPRY1, SPRY2, SPRY3, SPRY4, are central and complex regulators of the receptor tyrosine kinase (RTK) signalling pathway.
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