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SPIN1-Specific antibody

Cat.#: 115571

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Product Information

  • Product Name
    SPIN1-Specific antibody
  • Documents
  • Description
    SPIN1-Specific Rabbit Polyclonal antibody. Positive WB detected in SKOV-3 cells, A2780 cells, HEK-293 cells. Positive IF detected in Hela cells. Positive IHC detected in human ovary tumor tissue. Observed molecular weight by Western-blot: 30kd
  • Tested applications
    ELISA, WB, IHC, IF
  • Species reactivity
    Human; other species not tested.
  • Alternative names
    OCR antibody; Ovarian cancer related protein antibody; SPIN antibody; SPIN1 antibody; spindlin 1 antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of Peptide (Accession Number: NM_006717). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:500-1:5000

    IHC: 1:20-1:200

    IF: 1:10-1:100

  • Validations

    SKOV-3 cells were subjected to SDS PAGE followed by western blot with Catalog No:115571(SPIN1 antibody) at dilution of 1:500

    SKOV-3 cells were subjected to SDS PAGE followed by western blot with Catalog No:115571(SPIN1 antibody) at dilution of 1:500

    Immunohistochemical of paraffin-embedded human ovary tumor using Catalog No:115571(SPIN1 antibody) at dilution of 1:100 (under 10x lens)

    Immunohistochemical of paraffin-embedded human ovary tumor using Catalog No:115571(SPIN1 antibody) at dilution of 1:100 (under 10x lens)

    Immunohistochemical of paraffin-embedded human ovary tumor using Catalog No:115571(SPIN1 antibody) at dilution of 1:100 (under 40x lens)

    Immunohistochemical of paraffin-embedded human ovary tumor using Catalog No:115571(SPIN1 antibody) at dilution of 1:100 (under 40x lens)

    Immunofluorescent analysis of Hela cells, using SPIN1 antibody Catalog No:115571 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

    Immunofluorescent analysis of Hela cells, using SPIN1 antibody Catalog No:115571 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

  • Background
    Spindlin 1 (SPIN1) is a stage specific embryonic protein. It was reported as an abundant maternal transcript present in the unfertilized egg and 2-cell, but not 8-cell, stage embryo. Spindlin 1 was phosphorylated in a cell cycle-dependent manner, undergoing posttranslational modification during the first meiotic cycle. This antibody is specific to spindlin 1 (SPIN1), not to other spindlins, such as SPIN2 and SPIN3.
  • References
    • Sulpizio M, Falone S, Amicarelli F. Molecular basis underlying the biological effects elicited by extremely low-frequency magnetic field (ELF-MF) on neuroblastoma cells. Journal of cellular biochemistry. 112(12):3797-806. 2011.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"