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Product Name
SPI1 antibody
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Description
SPI1 Rabbit Polyclonal antibody. Positive IF detected in HepG2 cells. Positive WB detected in mouse liver tissue, HepG2 cells, RAW264.7 cells. Observed molecular weight by Western-blot: 31-38 kDa
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Tested applications
ELISA, WB, IF
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
31 kDa transforming protein antibody; PU.1 antibody; SFPI1 antibody; SPI 1 antibody; SPI A antibody; SPI1 antibody; Transcription factor PU.1 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of Peptide (Accession Number: NM_003120). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IF: 1:50-1:500
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Validations
mouse liver tissue were subjected to SDS PAGE followed by western blot with Catalog No:115568(SPI1 antibody) at dilution of 1:300
Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using Catalog No:115568(SPI1 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
SPI1 binds to the PU-box which is a purine-rich DNA sequence (5'-GAGGAA-3') that can act as a lymphoid-specific enhancer. SPI1 is a transcriptional activator that may be specifically involved in the differentiation or activation of macrophages or B-cells. It also binds RNA and may modulate pre-mRNA splicing. The antibody is specific to SPI1.
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References
- Lin HS, Gong JN, Su R. miR-199a-5p inhibits monocyte/macrophage differentiation by targeting the activin A type 1B receptor gene and finally reducing C/EBPα expression. Journal of leukocyte biology. 96(6):1023-35. 2014.
- Shi Y, Xu LZ, Peng K. Specific immunotherapy in combination with Clostridium butyricum inhibits allergic inflammation in the mouse intestine. Scientific reports. 5:17651. 2015.
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