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Product Name
SPHK2 antibody
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Description
SPHK2 Rabbit Polyclonal antibody. Positive WB detected in mouse cerebellum tissue, human kidney tissue, L02 cells, mouse brain tissue, mouse heart tissue, mouse kidney tissue, mouse liver tissue. Positive IP detected in mouse liver tissue. Positive IHC detected in human kidney tissue, human liver tissue. Observed molecular weight by Western-blot: 65 kDa,70 kDa
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Tested applications
ELISA, WB, IHC, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
SK 2 antibody; sphingosine kinase 2 antibody; SPHK2 antibody; SPK 2 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of SPHK2 recombinant protein (Accession Number: XM_006723292). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:2000
IHC: 1:20-1:200
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Validations
mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with Catalog No:115567(SPHK2 antibody) at dilution of 1:300
Immunohistochemical of paraffin-embedded human kidney using Catalog No:115567(SPHK2 antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human kidney using Catalog No:115567(SPHK2 antibody) at dilution of 1:50 (under 40x lens)
IP Result of anti-SPHK2 (IP:Catalog No:115567, 4ug; Detection:Catalog No:115567 1:800) with mouse liver tissue lysate 4000ug.
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Background
SPHK2(Sphingosine kinase 2) is also named as SK2,SPK2 and catalyzes the phosphorylation of sphingosine to form sphingosine 1-phosphate (SPP), a lipid mediator with both intra- and extracellular functions. SPHK2 associates with HDAC1 and HDAC2 in repressor complexes and is selectively enriched at the promoters of the genes encoding the cyclin-dependent kinase inhibitor p21 or the transcriptional regulator c-fos, where it enhanced local histone H3 acetylation and transcription.SphK2 is a ~66kDa protein that is most abundantly expressed in the liver and heart(PMID:19168577).
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