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Product Name
SPHK1-Phospho-Ser225 antibody
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Description
SPHK1-Phospho-Ser225 Rabbit Polyclonal antibody. Positive WB detected in L02 cells, human testis tissue. Observed molecular weight by Western-blot: 45-47kd
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Tested applications
ELISA, WB
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Species reactivity
Human; other species not tested.
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Alternative names
SK 1 antibody; sphingosine kinase 1 antibody; SPHK antibody; SPHK1 phopho antibody; SPHK1-Phospho-Ser225 antibody; SPK antibody; SPK 1 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of Peptide (Accession Number: NM_182965). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
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Validations
L02 cells were subjected to SDS PAGE followed by western blot with Catalog No:115566(SPHK1-Phospho-Ser225 antibody) at dilution of 1:500
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Background
SPHK1, also named as SPHK and SPK, catalyzes the phosphorylation of sphingosine to form sphingosine 1-phosphate (SPP), a lipid mediator with both intra- and extracellular functions. SPHK1 acts on D-erythro-sphingosine and to a lesser extent sphinganine, but not other lipids, such as D,L-threo-dihydrosphingosine, N,N-dimethylsphingosine, diacylglycerol, ceramide, or phosphatidylinositol. The antibody is specific to Ser(311)-phospho of SPHK1(isoform2) or Ser(225)-phospho of SPHK1(isoform1).
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"