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Product Name
Mouse SMPD1 (His Tag) recombinant protein
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Description
Converts sphingomyelin to ceramide. Also has phospholipase C activities toward 1,2-diacylglycerolphosphocholine and 1,2-diacylglycerolphosphoglycerol.
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Protein name
Sphingomyelin phosphodiesterase
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Protein short names
SMPD1; A-SMASE; NPD; ASM; ASMASE; ZN-SMASE
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Uniprot ID
Q04519
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Gene Name
Smpd1; Asm
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Source/Expression Host
Baculovirus-Insect Cells
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Expression Plasmid/cDNA
A DNA sequence encoding the mouse SMPD1 (Q04519) (Met 1-Leu 626) was expressed?with a C-terminal polyhistidine tag.
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Protein Species
Mouse
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Molecular weight
The secreted recombinant mouse SMPD1 consists of 592 amino acids and has a calculated molecular mass of 66.3 kDa. It migrates as an approximately 63 kDa band in SDS-PAGE under reducing conditions.
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Purity
> 85 % as determined by SDS-PAGE
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Activity
Measured by its ability to cleave 2-N-Hexadecanoylamino-4-nitrophenylphosphorylcholine(HNPPC).
The specific activity is > 1,500 pmoles/min/μg. -
Validations
Mouse SMPD1 / ASM Protein (His Tag) SDS-PAGE
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