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Product Name
SMPD1 / ASM Polyclonal Antibody
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Description
Polyclonal antibody to SMPD1 / ASM
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Tested applications
WB, IHC, IF
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Species reactivity
Human, Mouse, Rat
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Alternative names
SMPD1 antibody; ASM antibody; ASMASE antibody; NPD antibody; sphingomyelin phosphodiesterase antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 70-340 of human SMPD1 / ASM (NP_000534.3).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:10 - 1:100 -
Validations
Western blot - SMPD1 / ASM Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using SMPD1 / ASM antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.
Immunohistochemistry - SMPD1 / ASM Polyclonal Antibody
Immunohistochemistry of paraffin-embedded rat ovary using SMPD1 / ASM antibody at dilution of 1:100 (40x lens).
Immunofluorescence - SMPD1 / ASM Polyclonal Antibody
Immunofluorescence analysis of HeLa cells using SMPD1 / ASM antibody . Blue: DAPI for nuclear staining.
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Background
Converts sphingomyelin to ceramide. Also has phospholipase C activities toward 1,2-diacylglycerolphosphocholine and 1,2-diacylglycerolphosphoglycerol.; Isoform 2 lacks residues that bind the cofactor Zn(2+) and has no enzyme activity.; Isoform 3 lacks residues that bind the cofactor Zn(2+) and has no enzyme activity.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"