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Product Name
SPDEF Polyclonal Antibody
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Description
Polyclonal antibody to SPDEF
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Tested applications
WB, IHC, IF
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Species reactivity
Human, Mouse, Rat
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Alternative names
SPDEF antibody; PDEF antibody; bA375E1.3 antibody; SAM pointed domain-containing Ets transcription factor antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-335 of human SPDEF (NP_036523.1).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:1000
IHC 1:50 - 1:200
IF 1:50 - 1:100 -
Validations
Western blot - SPDEF Polyclonal Antibody
Western blot analysis of extracts of rat ovary, using SPDEF antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 30s.
Immunohistochemistry - SPDEF Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human placenta using SPDEF antibody at dilution of 1:100 (40x lens).
Immunofluorescence - SPDEF Polyclonal Antibody
Immunofluorescence analysis of MCF7 cells using SPDEF antibody .
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Background
May function as an androgen-independent transactivator of the prostate-specific antigen (PSA) promoter. Binds to 5'-GGAT-3' DNA sequences. May play a role in the regulation of the prostate gland and/or prostate cancer development. Acts as a transcriptional activator for SERPINB5 promoter.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"