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Product Name
SPCS1 antibody
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Description
SPCS1 Rabbit Polyclonal antibody. Positive WB detected in human skin tissue, BxPC-3 cells, PC-3 cells. Positive IF detected in BxPC-3 cells. Positive IHC detected in human prostate cancer tissue. Observed molecular weight by Western-blot: 12 kDa
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Tested applications
ELISA, WB, IHC, IF
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
HSPC033 antibody; SPase 12 kDa subunit antibody; SPC1 antibody; SPC12 antibody; SPCS1 antibody; YJR010C A antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of SPCS1 recombinant protein (Accession Number: BC000884). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
human skin tissue were subjected to SDS PAGE followed by western blot with Catalog No:115558(SPCS1 antibody) at dilution of 1:400
Immunohistochemical of paraffin-embedded human prostate cancer using Catalog No:115558(SPCS1 antibody) at dilution of 1:50 (under 10x lens)
Immunofluorescent analysis of BxPC-3 cells using Catalog No:115558(SPCS1 Antibody) at dilution of 1:25 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
SPCS1 represents signal peptidase complex subunit 1 homolog (S. cerevisiae)
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References
- Suzuki R, Matsuda M, Watashi K. Signal peptidase complex subunit 1 participates in the assembly of hepatitis C virus through an interaction with E2 and NS2. PLoS pathogens. 9(8):e1003589. 2013.
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