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Spartin, SPG20 antibody

Cat.#: 115545

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Product Information

  • Product Name
    Spartin, SPG20 antibody
  • Documents
  • Description
    Spartin, SPG20 Rabbit Polyclonal antibody. Positive IP detected in mouse brain tissue. Positive WB detected in NIH/3T3 cells, HEK-293 cells, HeLa cells, human brain tissue, mouse brain tissue, rat brain tissue, SH-SY5Y cells. Positive FC detected in HepG2 cells. Positive IHC detected in human gliomas tissue. Positive IF detected in HepG2 cells. Observed molecular weight by Western-blot: 75 kDa,84 kDa
  • Tested applications
    ELISA, IHC, IF, IP, WB, FC
  • Species reactivity
    Human,Mouse,Rat; other species not tested.
  • Alternative names
    KIAA0610 antibody; SPARTIN antibody; Spastic paraplegia 20 protein antibody; SPG20 antibody; TAHCCP1 antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of Spartin, SPG20 recombinant protein (Accession Number: NM_015087). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:500-1:5000

    IP: 1:500-1:5000

    IHC: 1:20-1:200

    IF: 1:10-1:100

  • Validations

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with Catalog No:115545(Spartin, SPG20 antibody) at dilution of 1:1000

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with Catalog No:115545(Spartin, SPG20 antibody) at dilution of 1:1000

    IP Result of anti-Spartin, SPG20 (IP:Catalog No:115545, 3ug; Detection:Catalog No:115545 1:1000) with mouse brain tissue lysate 6000ug.

    IP Result of anti-Spartin, SPG20 (IP:Catalog No:115545, 3ug; Detection:Catalog No:115545 1:1000) with mouse brain tissue lysate 6000ug.

    Immunohistochemical of paraffin-embedded human gliomas using Catalog No:115545(Spartin, SPG20 antibody) at dilution of 1:100 (under 10x lens)

    Immunohistochemical of paraffin-embedded human gliomas using Catalog No:115545(Spartin, SPG20 antibody) at dilution of 1:100 (under 10x lens)

    Immunohistochemical of paraffin-embedded human gliomas using Catalog No:115545(Spartin, SPG20 antibody) at dilution of 1:100 (under 40x lens)

    Immunohistochemical of paraffin-embedded human gliomas using Catalog No:115545(Spartin, SPG20 antibody) at dilution of 1:100 (under 40x lens)

    Immunofluorescent analysis of HepG2 cells, using SPG20 antibody Catalog No:115545 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

    Immunofluorescent analysis of HepG2 cells, using SPG20 antibody Catalog No:115545 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

    1X10^6 HepG2 cells were stained with 0.2ug Spartin, SPG20 antibody (Catalog No:115545, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

    1X10^6 HepG2 cells were stained with 0.2ug Spartin, SPG20 antibody (Catalog No:115545, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

  • Background
    SPG20 (spastic paraplegia 20) gene encodes a multifunctional Spartin protein. SPG20 protein is highly expressed in adipose tissue and may be implicated in endosomal trafficking and microtubule dynamics. SPG20 is mutated in Troyer syndrome, a hereditary spastic paraplegia defined by degeneration of upper motor neurons, recent study showed that regulation of SPG20 on mitochondrial calcium homeostasis may contribute to the pathophysiology of Troyer syndrome. Hypermethylation of SPG20 promoter, found in colorectal cancer paitents, may play a role in cytokinesis arrest in colorectal tumorigenesis.
  • References
    • Renvoisé B, Stadler J, Singh R, Bakowska JC, Blackstone C. Spg20-/- mice reveal multimodal functions for Troyer syndrome protein spartin in lipid droplet maintenance, cytokinesis and BMP signaling. Human molecular genetics. 21(16):3604-18. 2012.
    • Karlsson AB, Washington J, Dimitrova V, Hooper C, Shekhtman A, Bakowska JC. The role of spartin and its novel ubiquitin binding region in DALIS occurrence. Molecular biology of the cell. 25(8):1355-65. 2014.
    • Milewska M, Byrne PC. Different expression levels of spartin cause broad spectrum of cellular consequences in human neuroblastoma cells. Cell biology international. 39(9):1007-15. 2015.
    • Nicholson JM, Macedo JC, Mattingly AJ. Chromosome mis-segregation and cytokinesis failure in trisomic human cells. eLife. 4:. 2015.
    • Yan J, Zhang H, Liu Y. Germline deletion of huntingtin causes male infertility and arrested spermiogenesis in mice. Journal of cell science. 129(3):492-501. 2016.
    • Manzini MC, Rajab A, Maynard TM. Developmental and degenerative features in a complicated spastic paraplegia. Annals of neurology. 67(4):516-25. 2010.
    • Lind GE, Raiborg C, Danielsen SA. SPG20, a novel biomarker for early detection of colorectal cancer, encodes a regulator of cytokinesis. Oncogene. 30(37):3967-78. 2011.
    • Urbanczyk A, Enz R. Spartin recruits PKC-ζ via the PKC-ζ-interacting proteins ZIP1 and ZIP3 to lipid droplets. Journal of neurochemistry. 118(5):737-48. 2011.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"