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Product Name
SPARCL1 antibody
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Description
SPARCL1 Rabbit Polyclonal antibody. Positive IHC detected in human gliomas tissue. Positive IP detected in Raji cells. Positive WB detected in human heart tissue. Observed molecular weight by Western-blot: 130-140 kDa
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Tested applications
ELISA, WB, IHC, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
Hevin antibody; MAST 9 antibody; PIG33 antibody; SC1 antibody; SPARC like 1 (hevin) antibody; SPARC like protein 1 antibody; SPARCL1 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of SPARCL1 recombinant protein (Accession Number: NM_004684). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:1000
IHC: 1:20-1:200
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Validations
human heart tissue were subjected to SDS PAGE followed by western blot with Catalog No:115544(SPARCL1 antibody) at dilution of 1:400
IP Result of anti-SPARCL1 (IP:Catalog No:115544, 4ug; Detection:Catalog No:115544 1:300) with Raji cells lysate 1440ug.
Immunohistochemistry of paraffin-embedded human gliomas tissue slide using Catalog No:115544(SPARCL1 Antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemistry of paraffin-embedded human gliomas tissue slide using Catalog No:115544(SPARCL1 Antibody) at dilution of 1:50 (under 40x lens)
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Background
SPARC-like protein 1 (SPARCL1), also known as SC1 or Hevin, is an extracellular matrix glycoprotein that belongs to the SPARC family. SPARCL1 is widely expressed in normal and cancer tissues, and has been shown to regulate cellular adhesion and migration. SPARCL1 has been implicated in tumor initiation, formation, and progression of various cancers, including pancreatic, prostate, bladder, ovarian, breast and non-small cell lung cancers.
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