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Product Name
SPAG8 antibody
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Description
SPAG8 Rabbit Polyclonal antibody. Positive IF detected in PC-3 cells. Positive IHC detected in human prostate cancer tissue. Positive WB detected in PC-3 cells, DU 145 cells, human testis tissue, mouse testis tissue. Positive IP detected in mouse testis tissue. Observed molecular weight by Western-blot: 56-65 kDa
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Tested applications
ELISA, WB, IHC, IP, IF
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Species reactivity
Human, Mouse; other species not tested.
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Alternative names
BS 84 antibody; HSD 1 antibody; hSMP 1 antibody; SMP 1 antibody; SMP1 antibody; SPAG3 antibody; SPAG8 antibody; sperm assOCiated antigen 8 antibody; Sperm membrane protein 1 antibody; Sperm membrane protein BS 84 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of SPAG8 recombinant protein (Accession Number: NM_172312). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:50-1:500
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Validations
PC-3 cells were subjected to SDS PAGE followed by western blot with Catalog No:115536(SPAG8 antibody) at dilution of 1:1000
Immunohistochemical of paraffin-embedded human prostate cancer using Catalog No:115536(SPAG8 antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human prostate cancer using Catalog No:115536(SPAG8 antibody) at dilution of 1:100 (under 40x lens)
IP Result of anti-SPAG8 (IP:Catalog No:115536, 3ug; Detection:Catalog No:115536 1:500) with mouse testis tissue lysate 6000ug.
Immunofluorescent analysis of (-20oc Ethanol) fixed PC-3 cells using Catalog No:115536(SPAG8 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
SPAG8, also named as Sperm-associated antigen 8 or HSD-1, is a 426 amino acid protein, which is expressed in testis (germ cells), but not in liver, kidney, prostate and small intestine. SPAG8 may play a role in fertility and microtubule formation through interaction with RANBP9. SPAG8 as testis-specific protein is produced during male germ cell differentiation and functions in a germ cell type- and stage-specific manner during spermatogenesis.
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References
- Siliņa K, Zayakin P, Kalniņa Z. Sperm-associated antigens as targets for cancer immunotherapy: expression pattern and humoral immune response in cancer patients. Journal of immunotherapy (Hagerstown, Md. : 1997). 34(1):28-44. 2011.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"