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Product Name
SP100 antibody
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Description
SP100 Rabbit Polyclonal antibody. Positive IHC detected in human ovary tumor tissue. Positive WB detected in Jurkat cells. Observed molecular weight by Western-blot: 100 kDa
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Tested applications
ELISA, WB, IHC
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Species reactivity
Human; other species not tested.
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Alternative names
DKFZp686E07254 antibody; FLJ00340 antibody; FLJ34579 antibody; Lysp100b antibody; Nuclear autoantigen Sp 100 antibody; SP100 antibody; SP100 nuclear antigen antibody; Speckled 100 kDa antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of SP100 recombinant protein (Accession Number: NM_001206702). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IHC: 1:20-1:200
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Validations
Jurkat cells were subjected to SDS PAGE followed by western blot with Catalog No:115522(SP100 antibody) at dilution of 1:400
Immunohistochemical of paraffin-embedded human ovary tumor using Catalog No:115522(SP100 antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human ovary tumor using Catalog No:115522(SP100 antibody) at dilution of 1:50 (under 40x lens)
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Background
SP100 nuclear antigen, belongs to the nuclear bodies(NBs), has a role in the control of gene expression. It contains a HSR domain, which is important for the nuclear body targeting as well as for the dimerization, and one PxVxL motif, which if required for interaction with chromoshadow domains.
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References
- Full F, Jungnickl D, Reuter N. Kaposi's sarcoma associated herpesvirus tegument protein ORF75 is essential for viral lytic replication and plays a critical role in the antagonization of ND10-instituted intrinsic immunity. PLoS pathogens. 10(1):e1003863. 2014.
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