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SOD1 antibody

Cat.#: 115492

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Product Information

  • Product Name
    SOD1 antibody
  • Documents
  • Description
    SOD1 Rabbit Polyclonal antibody. Positive IF detected in HEK-293 cells. Positive IHC detected in human liver tissue, human brain tissue, human heart tissue, human kidney tissue, human lung tissue, human ovary tissue, human placenta tissue, human skin tissue, human spleen tissue, human testis tissue. Positive WB detected in HEK-293 cells, HeLa cells, human placenta tissue, mouse brain tissue, mouse liver tissue, rat brain tissue. Positive IP detected in HEK-293 cells. Observed molecular weight by Western-blot: 16-18 kDa
  • Tested applications
    ELISA, WB, IHC, IP, IF
  • Species reactivity
    Human,Mouse,Rat; other species not tested.
  • Alternative names
    ALS antibody; ALS1 antibody; homodimer antibody; IPOA antibody; SOD antibody; SOD1 antibody; Superoxide dismutase [Cu Zn] antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of SOD1 recombinant protein (Accession Number: NM_000454). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:500-1:5000

    IP: 1:500-1:5000

    IHC: 1:20-1:200

    IF: 1:10-1:100

  • Validations

    HEK-293 cells were subjected to SDS PAGE followed by western blot with Catalog No:115492(SOD1 antibody) at dilution of 1:1500

    HEK-293 cells were subjected to SDS PAGE followed by western blot with Catalog No:115492(SOD1 antibody) at dilution of 1:1500

    IP Result of anti-SOD1 (IP:Catalog No:115492, 3ug; Detection:Catalog No:115492 1:1500) with HEK-293 cells lysate 1000ug.

    IP Result of anti-SOD1 (IP:Catalog No:115492, 3ug; Detection:Catalog No:115492 1:1500) with HEK-293 cells lysate 1000ug.

    Immunohistochemical of paraffin-embedded human liver using Catalog No:115492(SOD1 antibody) at dilution of 1:100 (under 10x lens)

    Immunohistochemical of paraffin-embedded human liver using Catalog No:115492(SOD1 antibody) at dilution of 1:100 (under 10x lens)

    Immunohistochemical of paraffin-embedded human liver using Catalog No:115492(SOD1 antibody) at dilution of 1:100 (under 40x lens)

    Immunohistochemical of paraffin-embedded human liver using Catalog No:115492(SOD1 antibody) at dilution of 1:100 (under 40x lens)

    Immunofluorescent analysis of HEK-293 cells using Catalog No:115492(SOD1 Antibody) at dilution of 1:25 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

    Immunofluorescent analysis of HEK-293 cells using Catalog No:115492(SOD1 Antibody) at dilution of 1:25 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

  • Background
    Superoxide dismutase 1, soluble (amyotrophic lateral sclerosis 1 (adult)) (SOD1, synonyms: ALS, SOD, ALS1, IPOA) binds copper and zinc ions and is one of two isozymes responsible for destroying free uperoxide radicals in the body. This isozyme is a soluble cytoplasmic protein, acting as a homodimer to convert naturally-occuring but harmful superoxide radicals to molecular oxygen and hydrogen peroxide. The other isozyme is a mitochondrial protein. Mutations in this gene have been implicated as causes of familial amyotrophic lateral sclerosis.
  • References
    • Miyayama T, Suzuki KT, Ogra Y. Copper accumulation and compartmentalization in mouse fibroblast lacking metallothionein and copper chaperone, Atox1. Toxicology and applied pharmacology. 237(2):205-13. 2009.
    • Huang K, Huang J, Xie X. Sirt1 resists advanced glycation end products-induced expressions of fibronectin and TGF-β1 by activating the Nrf2/ARE pathway in glomerular mesangial cells. Free radical biology & medicine. 65:528-40. 2013.
    • Herrera C, Pettiglio MA, Bartnikas TB. Investigating the role of transferrin in the distribution of iron, manganese, copper, and zinc. Journal of biological inorganic chemistry : JBIC : a publication of the Society of Biological Inorganic Chemistry. 19(6):869-77. 2014.
    • Ackerman Z, Skarzinski G, Grozovski M, Oron-Herman M, Sela BA. Effects of antihypertensive and triglyceride-lowering agents on hepatic copper concentrations in rats with fatty liver disease. Basic & clinical pharmacology & toxicology. 115(6):545-51. 2014.
    • Huang K, Chen C, Hao J. Polydatin promotes Nrf2-ARE anti-oxidative pathway through activating Sirt1 to resist AGEs-induced upregulation of fibronetin and transforming growth factor-β1 in rat glomerular messangial cells. Molecular and cellular endocrinology. 399:178-89. 2015.
    • Aras S, Bai M, Lee I, Springett R, Hüttemann M, Grossman LI. MNRR1 (formerly CHCHD2) is a bi-organellar regulator of mitochondrial metabolism. Mitochondrion. 20:43-51. 2015.
    • Liu C, Lin JJ, Yang ZY, Tsai CC, Hsu JL, Wu YJ. Proteomic study reveals a co-occurrence of gallic acid-induced apoptosis and glycolysis in B16F10 melanoma cells. Journal of agricultural and food chemistry. 62(48):11672-80. 2014.
    • Xue H, Zhou S, Xiao L, Guo Q, Liu S, Wu Y. Hydrogen sulfide improves the endothelial dysfunction in renovascular hypertensive rats. Physiological research / Academia Scientiarum Bohemoslovaca. 64(5):663-72. 2015.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"