SOCS1 Polyclonal Antibody
Polyclonal antibody to SOCS1
Human, Mouse, Rat
SOCS1 antibody; CIS1 antibody; CISH1 antibody; JAB antibody; SOCS-1 antibody; SSI-1 antibody; SSI1 antibody; TIP3 antibody; TIP-3 antibody; suppressor of cytokine signaling 1 antibody
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-211 of human SOCS1 (NP_003736.1).
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Store at -20℃. Avoid freeze / thaw cycles.
WB 1:500 - 1:2000
IHC 1:50 - 1:200
Western blot - SOCS1 Polyclonal Antibody
Western blot analysis of extracts of mouse lung, using SOCS1 antibody at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 60s.
SOCS family proteins form part of a classical negative feedback system that regulates cytokine signal transduction. SOCS1 is involved in negative regulation of cytokines that signal through the JAK/STAT3 pathway. Through binding to JAKs, inhibits their kinase activity. In vitro, also suppresses Tec protein-tyrosine activity. Appears to be a major regulator of signaling by interleukin 6 (IL6) and leukemia inhibitory factor (LIF). Regulates interferon-gamma mediated sensory neuron survival (By similarity). Probable substrate recognition component of an ECS (Elongin BC-CUL2/5-SOCS-box protein) E3 ubiquitin ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Seems to recognize JAK2. SOCS1 appears to be a negative regulator in IGF1R signaling pathway.
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