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Product Name
SNX17 antibody
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Description
SNX17 Rabbit Polyclonal antibody. Positive IP detected in HeLa cells. Positive WB detected in human brain tissue, HeLa cells, HepG2 cells, mouse brain tissue, mouse cerebellum tissue, mouse liver tissue, mouse skin tissue. Positive IHC detected in human brain tissue. Positive IF detected in HepG2 cells. Observed molecular weight by Western-blot: 50 kDa
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Tested applications
ELISA, WB, IF, IHC, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
KIAA0064 antibody; SNX17 antibody; sorting nexin 17 antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of SNX17 recombinant protein (Accession Number: NM_014748). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
human brain tissue were subjected to SDS PAGE followed by western blot with Catalog No:115472(SNX17 antibody) at dilution of 1:300
Immunofluorescent analysis of HepG2 cells, using SNX17 antibody Catalog No:115472 at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
IP Result of anti-SNX17 (IP:Catalog No:115472, 4ug; Detection:Catalog No:115472 1:1000) with HeLa cells lysate 1200ug.
Immunohistochemistry of paraffin-embedded human brain tissue slide using Catalog No:115472(SNX17 Antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemistry of paraffin-embedded human brain tissue slide using Catalog No:115472(SNX17 Antibody) at dilution of 1:50 (under 40x lens)
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Background
Sorting nexin 17 (SNX17, synonym: KIAA0064) is a member of the sorting nexin family. Members of this family contain a phox (PX) domain, which is a phosphoinositide binding domain, and are involved in intracellular trafficking. SNX17 does not contain a coiled coil region, like some family members, but contains a B41 domain. This protein interacts with the cytoplasmic domain of P-selectin, and may function in the intracellular trafficking of P-selectin.
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References
- Adachi H, Tsujimoto M. Adaptor protein sorting nexin 17 interacts with the scavenger receptor FEEL-1/stabilin-1 and modulates its expression on the cell surface. Biochimica et biophysica acta. 1803(5):553-63. 2010.
- Böttcher RT, Stremmel C, Meves A. Sorting nexin 17 prevents lysosomal degradation of β1 integrins by binding to the β1-integrin tail. Nature cell biology. 14(6):584-92. 2012.
- Schorch B, Song S, van Diemen FR. LRP1 is a receptor for Clostridium perfringens TpeL toxin indicating a two-receptor model of clostridial glycosylating toxins. Proceedings of the National Academy of Sciences of the United States of America. 111(17):6431-6. 2014.
- Osborne DG, Piotrowski JT, Dick CJ, Zhang JS, Billadeau DD. SNX17 affects T cell activation by regulating TCR and integrin recycling. Journal of immunology (Baltimore, Md. : 1950). 194(9):4555-66. 2015.
- Ketel K, Krauss M, Nicot AS. A phosphoinositide conversion mechanism for exit from endosomes. Nature. 529(7586):408-12. 2016.
- Tseng HY, Thorausch N, Ziegler T, Meves A, Fässler R, Böttcher RT. Sorting nexin 31 binds multiple β integrin cytoplasmic domains and regulates β1 integrin surface levels and stability. Journal of molecular biology. 426(18):3180-94. 2014.
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